Ablation of UCP-1+ cells impacts FAP dynamics in muscle regeneration.

Uncoupling protein-1 (UCP-1+) cells found in brown adipose tissue and subtypes of white (a.k.a. beige) adipose tissue have been a focus of intensive investigation for their role in energy metabolism and are emerging as potential endocrine regulators of physiology. More recently, UCP-1+ subpopulations have also been found in skeletal muscle fibro-adipogenic progenitors (FAPs), which play an important role in regeneration. Both UCP-1+ adipocytes and FAPs secrete promyogenic cytokines further supporting their potential for proregenerative signaling. To investigate whether signaling from UCP-1+ cells does indeed promote regeneration, we examined injury-induced muscle regeneration in a mouse model with constitutive UCP-1+ cell ablation (UCP1-DTA) at three time points: early [3 and 7 days post injury (dpi)], intermediate (14 dpi), and late (21 dpi). We hypothesized that without UCP-1+ cells, muscle regeneration would be impaired at all time points. At 3 and 7 dpi, we found significantly reduced numbers of FAPs in male UCP1-DTA mice, but with no accompanying changes in muscle-derived stem (satellite) cells or immune cells. However, at 14 dpi, we observed significantly higher numbers of FAP in male UCP1-DTA mice and evidence of ongoing early-phase regeneration, including significantly increased histological and gene expression of early regenerative markers and significantly smaller regenerating fibers. However, these changes were not associated with fibrosis and fatty infiltration typical of impaired regeneration, nor were differences in contractile force recovery observed between genotypes. These findings suggest that UCP-1+ cells (adipocytes or FAPs) may regulate FAP dynamics in early regeneration, but without major effects on the recovery of structure and function.