Evidence map›Paper›PMID 40722982›Full record

ArticleAntioxidants (Basel, Switzerland)2025

Anti-Inflammatory Pathways Modulated by Microbial Polysaccharides from Euganean Thermal Muds in Zebrafish.

Micol Caichiolo, Raffaella Margherita Zampieri, Francesca Terrin, Annachiara Tesoriere, Fabrizio Caldara, Nicoletta La Rocca, Paolo Martini, Luisa Dalla Valle

Abstract read
In one paragraph

Article in Antioxidants (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Micol CaichioloDepartment of Biology, University of Padova, Via U. Bassi 58/b, 35131 Padova, Italy.ORCID 0000-0002-9941-0231
Raffaella Margherita ZampieriDepartment of Biology, University of Padova, Via U. Bassi 58/b, 35131 Padova, Italy.ORCID 0000-0002-4245-8699
Francesca TerrinDepartment of Biology, University of Padova, Via U. Bassi 58/b, 35131 Padova, Italy.ORCID 0000-0002-3484-1946
Annachiara TesoriereDepartment of Biology, University of Padova, Via U. Bassi 58/b, 35131 Padova, Italy.
Fabrizio CaldaraPietro d'Abano Thermal Studies Center, Via Jappelli 5, 35031 Padova, Italy.
Nicoletta La RoccaDepartment of Biology, University of Padova, Via U. Bassi 58/b, 35131 Padova, Italy.ORCID 0000-0003-4866-5952
Paolo MartiniDepartment of Molecular and Translational Medicine, University of Brescia, Viale Europa 11, 25123 Brescia, Italy.ORCID 0000-0002-0146-1031
Luisa Dalla ValleDepartment of Biology, University of Padova, Via U. Bassi 58/b, 35131 Padova, Italy.ORCID 0000-0001-8097-6369

Funding

Department of Biology, University of Padova Intramural GrantPietro d'Abano Thermal Studies Center DALL_PRIV22_01
6 · The paper itself

Abstract

Thermal mud produced by spas of the Euganean Thermal District (Italy) has been used since ancient times for therapeutic purposes. Recently, the anti-inflammatory activity of microbial polysaccharides (M-PS), extracted from traditionally maturated muds, was demonstrated using the zebrafish model organism. However, the downstream signalling pathways regulated by M-PS remain largely unknown. In this study, to investigate the underlying mechanisms of inflammation resolution, we performed a transcriptome analysis on zebrafish larvae inflamed with copper sulphate and treated with M-PS. Our findings revealed that M-PS treatment down-regulated the expression of key genes involved in several inflammatory pathways. Gene Set Enrichment Analysis identified eleven up-regulated pathways (e.g., TNF-α signalling via NFκB, IL6-JAK-STAT signalling, p53 pathway, apoptosis, and interferon response) with components reduced in number and expression level in M-PS-treated larvae compared to the inflamed ones. Additionally, seven down-regulated pathways were identified (e.g., transcription factors E2F, MYC, and the G2M checkpoint). DEG-pseudotime analysis further confirmed the association of these genes with the pathways identified by GSEA. These results provide valuable insights into the anti-inflammatory properties of M-PS and the therapeutic potential of Euganean thermal muds for inflammatory diseases.

Indexed as

bioactive moleculescopper sulphateinflammationmicrobial polysaccharidespeloidsthermal mudtranscriptomezebrafish

Identifiers

PMID40722982
PMCPMC12292280

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.