Evidence map›Paper›PMID 40723171›Full record

ArticleCancers2025

Analysis of Phenotypic and Molecular Variability of Memory-like NK Cells for Cancer Adoptive Cell Therapy Screening.

Rithvik V Turaga, Seth R T Zima, Ella P Peterson, Amy K Erbe, Matthew H Forsberg, Christian M Capitini, Pippa F Cosper, Paul M Sondel, Jose M Ayuso

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancers, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Rithvik V TuragaDepartment of Dermatology, School of Medicine and Public Health, University of Wisconsin, 1 S Park Street, Madison, WI 53715, USA.ORCID 0009-0009-2118-3444
Seth R T ZimaDepartment of Dermatology, School of Medicine and Public Health, University of Wisconsin, 1 S Park Street, Madison, WI 53715, USA.ORCID 0009-0007-9329-0068
Ella P PetersonDepartment of Dermatology, School of Medicine and Public Health, University of Wisconsin, 1 S Park Street, Madison, WI 53715, USA.ORCID 0009-0003-9183-4202
Amy K ErbeUW Carbone Cancer Center, 600 Highland Avenue, Madison, WI 53705, USA.
Matthew H ForsbergDepartment of Pediatrics, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53705, USA.ORCID 0000-0002-2276-6731
Christian M CapitiniUW Carbone Cancer Center, 600 Highland Avenue, Madison, WI 53705, USA.ORCID 0000-0003-3362-5587
Pippa F CosperUW Carbone Cancer Center, 600 Highland Avenue, Madison, WI 53705, USA.ORCID 0000-0002-0981-8875
Paul M SondelUW Carbone Cancer Center, 600 Highland Avenue, Madison, WI 53705, USA.
Jose M AyusoDepartment of Dermatology, School of Medicine and Public Health, University of Wisconsin, 1 S Park Street, Madison, WI 53715, USA.

Funding

UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · UNIVERSITY OF WISCONSIN-MADISON · 1985 to 2025
$35.4M
Project 3: Modulation of the head and neck tumor immune microenvironment by targeting the TAM family of receptorsP50CA278595 · UNIVERSITY OF WISCONSIN-MADISON · 2025 to 2025
$2.1M
Chromosomal Instability as a Marker and Mechanism of Radiation ResponseK08CA256166 · UNIVERSITY OF WISCONSIN-MADISON · 2025 to 2025
$185k
Department of Dermatology, UW-Madison naMidwest Athletes Against Childhood Cancer Fund naNCI NIH HHS K08 CA256166NCI NIH HHS K08CA256166NCI NIH HHS P30 CA014520NCI NIH HHS P50 CA278595NIDCR NIH HHS P50 DE026787Skin Disease Research Center, UW-Madison naSpecialized Program of Research Excellence (SPORE) through NIH National Institute for Dental and Craniofacial Research (NIDCR) and National Cancer Institute (NCI) P50DE026787St. Baldrick's Foundation Empowering Pediatric Immunotherapy for Childhood Cancers Team na
6 · The paper itself

Abstract

backgroundAdoptive cell therapies are emerging as a promising therapeutic option against hematological and solid malignancies. Memory-like natural killer (mlNK) cells are a specific subtype of NK cells generated after cytokine preactivation that have shown enhanced in vivo persistence after infusion into patients, an issue that has hindered traditional NK cell immunotherapy. However, the quality and variability of mlNK cell products remains poorly defined.

methodsIn this study, we evaluated heterogeneity across critical functional and molecular aspects of mlNK cells generated from independent donors, including mlNK cytotoxicity, cluster formation, motility, mitochondria morphology, and gene expression.

resultsWe observed a correlation between changes in gene expression associated with glycolysis and key NK cell functions such as cytotoxicity and motility. For further characterization, we blocked glycolysis and oxidative phosphorylation (OXPHOS) and observed an impaired mlNK functional response, suggesting the importance of metabolism.

conclusionsOur findings provide insights into discriminating between mlNK cell products and how the predictive markers can identify optimal mlNK cell products for adoptive cell therapy of cancer.

Indexed as

adoptive cell therapyhead and neck squamous cell carcinomaimmunotherapymemory-like natural killer cellsnatural killer cellsphenotypic and molecular variabilityprediction

Identifiers

PMID40723171
PMCPMC12293829

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.