Evidence map›Paper›PMID 40724989›Full record

ArticleInternational journal of molecular sciences2025

Acute Hyperglycemia-Induced Inflammation in MIO-M1 Cells: The Role of Aldose Reductase.

Francesca Felice, Gemma Sardelli, Francesco Balestri, Lucia Piazza, Mario Cappiello, Rossella Mosca, Antonella Del Corso, Martina Avanatti, Simone Allegrini, Roberta Moschini

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. ExploringMolecules (Basel, Switzerland) · 2026
    Article
  4. Article
  5. Article
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Francesca FeliceBiochemistry Unit, Department of Biology, University of Pisa, 56123 Pisa, Italy.ORCID 0000-0001-8968-2228
Gemma SardelliNational Research Council-CNR, Institute on Clinical Physiology, via Moruzzi 1, 56124 Pisa, Italy.ORCID 0000-0002-3617-0496
Francesco BalestriBiochemistry Unit, Department of Biology, University of Pisa, 56123 Pisa, Italy.
Lucia PiazzaBiochemistry Unit, Department of Biology, University of Pisa, 56123 Pisa, Italy.
Mario CappielloBiochemistry Unit, Department of Biology, University of Pisa, 56123 Pisa, Italy.ORCID 0000-0002-8864-5043
Rossella MoscaBiochemistry Unit, Department of Biology, University of Pisa, 56123 Pisa, Italy.
Antonella Del CorsoBiochemistry Unit, Department of Biology, University of Pisa, 56123 Pisa, Italy.ORCID 0000-0003-3165-6128
Martina AvanattiBiochemistry Unit, Department of Biology, University of Pisa, 56123 Pisa, Italy.ORCID 0009-0005-6326-2897
Simone AllegriniBiochemistry Unit, Department of Biology, University of Pisa, 56123 Pisa, Italy.ORCID 0000-0002-7005-654X
Roberta MoschiniBiochemistry Unit, Department of Biology, University of Pisa, 56123 Pisa, Italy.ORCID 0000-0001-5227-2348

Funding

Institution, University of Pisa 167-Fondi di ateneo 2023
6 · The paper itself

Abstract

Diabetic retinopathy (DR), traditionally considered a microvascular complication, is now recognized as a neuroinflammatory disorder involving retinal glial cells. Aldose reductase (AKR1B1), a key enzyme in the polyol pathway, has been implicated in the hyperglycemia-induced inflammatory response in various cell types, although its role in retinal Müller glial cells under acute glucose stress remains unclear. This study investigates AKR1B1 activity and its contribution to inflammatory signaling in MIO-M1 human Müller cells exposed to acute hyperglycemia. AKR1B1 expression and activity, as well as NF-κB activation and COX-2 expression, were evaluated. Sorbinil, a specific AKR1B1 inhibitor, was used to determine the enzyme's contribution to acute hyperglycemia-induced inflammation. Acute high-glucose treatment significantly increased AKR1B1 activity and sorbitol accumulation without affecting cell viability. In addition, activation of NF-κB and increased expression of cyclooxygenase-2 (COX-2) were observed, both of which were significantly reduced by Sorbinil. Our findings highlight the role of macroglia as active contributors to early inflammatory events in DR and suggest that transient hyperglycemic spikes are sufficient to trigger AKR1B1-dependent glial activation.

Indexed as

Aldehyde ReductaseEpendymoglial CellsHyperglycemiaInflammationCell LineCell SurvivalCyclooxygenase 2Diabetic RetinopathyGlucoseHumansImidazolidinesNF-kappa BSignal TransductionSorbitolAKR1B1 protein, humanAldehyde ReductaseCyclooxygenase 2GlucoseImidazolidinesNF-kappa BsorbinilSorbitolAKR1B1inflammationmacrogliaMIO-M1 cells

Identifiers

PMID40724989
PMCPMC12295778

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.