Evidence mapPaperPMID 40725215Full record

ArticleInternational journal of molecular sciences2025

Cryopreservation and Validation of Microfragmented Adipose Tissue for Autologous Use in Knee Osteoarthritis Treatment.

Marija Zekušić, Petar Brlek, Lucija Zenić, Vilim Molnar, Maja Ledinski, Marina Bujić Mihica, Adela Štimac, Beata Halassy, Snježana Ramić, Dominik Puljić and 6 more

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Marija ZekušićDepartment of Transfusion and Regenerative Medicine, Sestre Milosrdnice University Hospital Center, 10000 Zagreb, Croatia.
Petar BrlekDepartment of Molecular Biology, Faculty of Science, University of Zagreb, 10000 Zagreb, Croatia.ORCID 0000-0001-8022-4095
Lucija ZenićGreyledge Europe Ltd., 10000 Zagreb, Croatia.
Vilim MolnarSt. Catherine Specialty Hospital, 10000 Zagreb, Croatia.
Maja LedinskiDepartment of Transfusion and Regenerative Medicine, Sestre Milosrdnice University Hospital Center, 10000 Zagreb, Croatia.
Marina Bujić MihicaDepartment of Transfusion and Regenerative Medicine, Sestre Milosrdnice University Hospital Center, 10000 Zagreb, Croatia.
Adela ŠtimacCentre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, 10000 Zagreb, Croatia.
Beata HalassyCentre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, 10000 Zagreb, Croatia.ORCID 0000-0001-7370-0997
Snježana RamićDepartment of Pathology and Cytology 'Ljudevit Jurak', Sestre Milosrdnice University Hospital Center, 10000 Zagreb, Croatia.
Dominik PuljićDepartment of Transfusion and Regenerative Medicine, Sestre Milosrdnice University Hospital Center, 10000 Zagreb, Croatia.
Tiha VučemiloDepartment of Transfusion and Regenerative Medicine, Sestre Milosrdnice University Hospital Center, 10000 Zagreb, Croatia.
Carlo TremoladaImage Regenerative Clinic, 20122 Milan, Italy.ORCID 0000-0003-1331-5995
Srećko SabalićDepartment of Traumatology, Sestre Milosrdnice University Hospital Center, 10000 Zagreb, Croatia.
David C KarliGreyledge Technologies, LLC, Miami, FL 33133, USA.
Dimitrios TsoukasOrthopaedic Clinic for Advanced Arthroscopic Sports and Regenerative Surgery Mitera Hospital, 15123 Athens, Greece.ORCID 0000-0001-7966-6670
Dragan PrimoracSt. Catherine Specialty Hospital, 10000 Zagreb, Croatia.ORCID 0000-0001-5565-080X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Micro-fragmented adipose tissue (MFAT) is a promising autologous therapy for knee osteoarthritis. To avoid repeated liposuction procedures for its clinical application, MFAT obtained from patients with knee osteoarthritis was stored at -80 °C in a tissue bank. This study describes the preparation, cryopreservation, thawing, and washing, as well as comprehensive analysis of cell populations in fresh and MFAT thawed after two years. Immunophenotyping of both fresh and thawed MFAT showed a significant presence of endothelial progenitors and pericytes in the stromal vascular fraction. Viability before (59.75%) and after freezing (55.73%) showed no significant difference. However, the average cell count per gram of MFAT was significantly reduced in thawed samples (3.00 × 10

Indexed as

Adipose TissueCryopreservationOsteoarthritis, KneeAdipocytesAgedFemaleHumansImmunophenotypingMaleMiddle AgedTransplantation, Autologousadipose tissueendothelial progenitor cellMFATosteoarthritispericyte

Identifiers

PMID40725215
PMCPMC12295665

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.