Evidence map›Paper›PMID 40727716›Full record

ArticleOptica2025

High-speed 4D fluorescence light field tomography of whole freely moving organisms.

Kevin C Zhou, Clare Cook, Archan Chakraborty, Jennifer Bagwell, Joakim Jönsson, Kyung Chul Lee, Xi Yang, Shiqi Xu, Ramana Balla, Kushal Kolar and 5 more

Abstract read
In one paragraph

Article in Optica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Kevin C ZhouDepartment of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Clare CookDepartment of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Archan ChakrabortyDepartment of Pharmacology & Cancer Biology, Duke University, Durham, North Carolina, USA.
Jennifer BagwellDepartment of Cell Biology, Duke University, Durham, North Carolina, USA.
Joakim JönssonDepartment of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Kyung Chul LeeDepartment of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Xi YangDepartment of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Shiqi XuDepartment of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Ramana BallaDepartment of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Kushal KolarDepartment of Biomedical Engineering, Tandon School of Engineering, New York University, Brooklyn, New York, USA.
Caitlin LewisDepartment of Electrical & Computer Engineering, Duke University, Durham, North Carolina, USA.
Mark HarfoucheRamona Optics Inc., 1000 W Main St., Durham, North Carolina 27701, USA.
Donald T FoxDepartment of Pharmacology & Cancer Biology, Duke University, Durham, North Carolina, USA.
Michel BagnatDepartment of Cell Biology, Duke University, Durham, North Carolina, USA.
Roarke HorstmeyerDepartment of Biomedical Engineering, Duke University, Durham, North Carolina, USA.

Funding

Parallelized Imaging and Automated Analysis of Zebrafish Assays with a Gigapixel MicroscopeR44OD024879 · OD · RAMONA OPTICS, INC. · PI HARFOUCHE, MARK · 2018 to 2022
$3.5M
Polyploidy after tissue injury: a Drosophila modelR01GM118447 · NIGMS · DUKE UNIVERSITY · PI FOX, DONALD T. · 2016 to 2025
$3.1M
Rapid 3D Whole-Slide Digitization of Thick Cytopathology Slides with a Gigapixel MicroscopeR44CA250877 · NCI · RAMONA OPTICS, INC. · PI HARFOUCHE, MARK · 2020 to 2022
$2.4M
High-content behavioral phenotyping of model organisms in three dimensions with a gigapixel microscopeR44OD036187 · OD · RAMONA OPTICS, INC. · PI HARFOUCHE, MARK · 2024 to 2025
$2.0M
Investigating mechanisms of cell volume regulation in the zebrafish notochordR01GM150666 · NIGMS · DUKE UNIVERSITY · PI Michel Bagnat · 2024 to 2026
$1.3M
Broken chromosome segregation during mitosis: a Drosophila modelR01GM140138 · NIGMS · DUKE UNIVERSITY · PI FOX, DONALD T. · 2022 to 2025
$1.2M
A wide-field, high-resolution 3D exoscope for improved microsurgery workflowR43EB030979 · NIBIB · RAMONA OPTICS, INC. · PI HARFOUCHE, MARK · 2020 to 2020
$252k
High-Resolution, Parallelized Imaging of Freely Swimming Zebrafish with a Gigapixel MicroscopeR43OD024879 · OD · RAMONA OPTICS, INC. · PI HARFOUCHE, MARK, HORSTMEYER, ROARKE · 2017 to 2017
$225k
NCI NIH HHS R44 CA250877NIBIB NIH HHS R43 EB030979NIGMS NIH HHS R01 GM118447NIGMS NIH HHS R01 GM140138NIGMS NIH HHS R01 GM150666NIH HHS R43 OD024879NIH HHS R44 OD024879NIH HHS R44 OD036187
6 · The paper itself

Abstract

Volumetric fluorescence imaging techniques, such as confocal, multiphoton, light sheet, and light field microscopy, have become indispensable tools across a wide range of cellular, developmental, and neurobiological applications. However, it is difficult to scale such techniques to the large 3D fields of view (FOV), volume rates, and synchronicity requirements for high-resolution 4D imaging of freely behaving organisms. Here, we present reflective Fourier light field computed tomography (ReFLeCT), a high-speed volumetric fluorescence computational imaging technique. ReFLeCT synchronously captures entire tomograms of multiple unrestrained, unanesthetized model organisms across multi-millimeter 3D FOVs at 120 volumes per second. In particular, we applied ReFLeCT to reconstruct 4D videos of fluorescently labeled zebrafish and

Identifiers

PMID40727716
PMCPMC12302686

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.