ArticleInvestigative ophthalmology & visual science2025
Calcitonin Gene-Related Peptide Regulates Specific Interferon-Stimulating Genes to Inhibit Apoptosis of Corneal Epithelial Cells in Dry Eye Disease.
Article in Investigative ophthalmology & visual science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Protective Effect of Calcitonin Gene-Related Peptide on Corneal Epithelial Barrier in Experimental Dry Eye Models.Investigative ophthalmology & visual science · 2026Article
- Corneal Nerves Promote Alkali Burn Repair by Modulating Macrophages and Neutrophils via Calcitonin Gene-Related Peptide.Investigative ophthalmology & visual science · 2026Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose: Calcitonin gene-related peptide (CGRP) has demonstrated the potential to treat dry eye disease (DED), although its mechanism is not well-understood. This study investigated the effect of CGRP on apoptosis of corneal epithelial cells in a model of DED. Methods: Tear samples were collected from patients with DED. Additionally, samples were collected from a rat model of DED and a CGRP overexpression human corneal epithelial (HCET) model. The concentrations of CGRP and IFN-γ in tears were detected by ELISA and analyzed in combination with clinical data. The apoptosis level of corneal epithelial cells was evaluated by Western blot, qRT-PCR, TUNEL staining, and flow cytometry. RNA-seq screening and validation of CGRP target proteins in corneal epithelial cells was also performed. Results: The findings of this study demonstrated a significant and positive correlation between CGRP expressed in tears of patients with DED and multiple clinical indicators of DED (P < 0.05). CGRP expression was similarly increased in the DED animal model. Exogenous CGRP peptides were observed to significantly inhibit the apoptosis of corneal tissue (P < 0.05). The CGRP overexpressing group similarly showed decreased levels of apoptosis in the corneal tissue (P < 0.05). Reduced apoptosis of corneal tissues was associated with the IFN-γ/JAK2/STAT1 pathway. RNA-seq suggested that CGRP(8-37) concurrently increased apoptosis, inhibited the expression of specific interferon-stimulating genes (SISGs), and was related to the activation of IFN-γ/JAK2/STAT1 (P < 0.05). Conclusions: High CGRP concentration in tears of patients with DED demonstrated a significant correlation with severity of clinical symptoms. Increased CGRP was associated with reduced apoptosis of corneal epithelial cells in an animal model of DED. Molecular evaluation identified inhibition of SISGs and activation of IFN-γ/JAK2/STAT1 as CGRP-related mechanisms potentially mediating reduced apoptosis.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.