Yeast Oral Delivery of DAF16 shRNAs Results in Effective Gene Silencing in

Benedetta Caraba, Arianna Montanari, Emily Schifano, Fabiana Stocchi, Giovanna Costanzo, Daniela Uccelletti, Cristina Mazzoni

Abstract read

PubMed Full text at PMC Publisher

In one paragraph

Article in Current issues in molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Benedetta CarabaDepartment of Biology and Biotechnologies "C. Darwin", Sapienza University of Rome, 00185 Rome, Italy.ORCID 0009-0005-0609-3679

Arianna MontanariDepartment of Biology and Biotechnologies "C. Darwin", Sapienza University of Rome, 00185 Rome, Italy.ORCID 0000-0001-6832-7569

Emily SchifanoDepartment of Biology and Biotechnologies "C. Darwin", Sapienza University of Rome, 00185 Rome, Italy.

Fabiana StocchiDepartment of Biology and Biotechnologies "C. Darwin", Sapienza University of Rome, 00185 Rome, Italy.

Giovanna CostanzoInstitute of Molecular Biology and Pathology, National Research Council (IBPM-CNR), 00185 Rome, Italy.ORCID 0000-0002-9494-5536

Daniela UccellettiDepartment of Biology and Biotechnologies "C. Darwin", Sapienza University of Rome, 00185 Rome, Italy.ORCID 0000-0001-6841-8129

Cristina MazzoniDepartment of Biology and Biotechnologies "C. Darwin", Sapienza University of Rome, 00185 Rome, Italy.ORCID 0000-0002-1504-6189

Funding

Sapienza University of Rome grant number RM1241907D0F9C7B and RG12218166E3EE68

6 · The paper itself

Abstract

Plant Parasitic Nematodes (PPNs) are a major problem in agriculture. Damage caused by PPNs has been estimated at USD 80-157 billion annually. The estimates could be even worse in the future in the context of a growing world population in a climate change scenario and with the removal/reduction in the use of some nematodicides due to the strong ecological impact. Biocontrol Agents (BCAs) currently constitute only 8.8% of the general pesticide market. With a view to an ecological transition, the transition from pesticides to biopesticides represents an important challenge that appears necessary not only for organic farming, but also in so-called integrated agriculture. Among the possible BCAs, microorganisms, and in particular yeast, which enjoys the GRAS (Generally Recognized As Safe) status, have the advantage of being able to be produced on a large scale by fermentation on waste substrates at low cost. In this paper, as proof of concept we constructed yeast strains expressing short hairpin RNAs (shRNAs) targeting the

Indexed as

Biocontrol Agentnematodesoral deliveryRNA interferenceyeast

Identifiers

PMID40729039

DOI10.3390/cimb47070570

PMCPMC12293383

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.