ArticleReproductive biology and endocrinology : RB&E2025
Impact of aging on gene expression in human oocytes: a comparative analysis of young and older patients.
Article in Reproductive biology and endocrinology : RB&E, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed.
- Endocrine and Molecular Mechanisms of Oocyte Aging: From Gonadotropin Signaling to Metabolic and Microenvironmental Dysfunction.Current issues in molecular biology · 2026Review
- Maternal age as a driver of genome instability: mechanisms linking aneuploidy, mutagenesis and mitochondrial dysfunction.Archives of toxicology · 2026Review
- Genome-Wide Integration Site Profiling of Multi-Component CAR T-Cell Engineering Systems with ADA1/CD26 Co-Transduction.Biomedicines · 2026Article
- Expert consensus on the detection and clinical application of tumor mutational burden.Cancer biology & medicine · 2026Article
- In Vitro Fertilization Accelerates Female Reproductive Aging Through Early Ovarian Failure.bioRxiv : the preprint server for biology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAging affects gene expression in pathways essential for energy metabolism, DNA repair, cell cycle regulation, and antioxidant defenses, directly affecting oocyte quality and viability. Single-cell RNA deep sequencing studies of aged versus young human MII oocytes revealed many differentially expressed genes. In addition, single human oocyte transcriptome analysis at both germinal vesicle (GV) and MII stages revealed distinct stage-dependent pathways impacted by aging, with a decrease in mitochondrial-related transcripts from GV to MII oocytes, and a much greater reduction in MII oocytes with advanced age.
objectiveOur aim was to investigate the age-related differences in gene expression of germinal vesicle (GV) oocytes between young and advanced age patients. PATIENTS AND
methodsImmature GV oocytes were donated by 6 patients, divided into two age groups: The "Young" group (ages 16-29) had three participants (mean age: 23.3 ± 6.6 years), and the "Elderly" group (ages 38-40) included three participants (mean age: 39 ± 1 year). After retrieval, oocytes were denuded and donated GV oocytes were cryopreserved at -196
resultsOf top 10 significantly differently expressed genes 7 (LINC02087, POMZP3, LINC02749, MYL4, AGPAT2, GCA, and LIMK1) were downregulated and 3 (CLEC3A, ARPP21, and CITED2) showed significant upregulation in young versus old oocytes. These genes underscore the impact of aging on critical oocyte pathways, including chromosomal stability, epigenetic regulation, mitochondrial function, immune response, structural integrity, and calcium signaling. Moreover, among these genes, LINC02087 was the most downregulated (log2FC = -7.66), while CITED2 showed the strongest upregulation (log2FC = 3.43) in young versus old oocytes. Following the RNA extraction of pooled GV oocytes of 8 elderly and 9 young donors' GV oocytes. We observed significant differences in gene expression levels between the two age groups, in line with the single-cell RNASeq.
conclusionUnderstanding the effects of aging on the oocyte transcriptome could identify biomarkers that characterize good MII oocyte quality. The different genes expressions in aged oocytes highlight their potential contributions to oocyte quality and development. Moreover, by elucidating age-related changes across diverse cellular functions, this preliminary study opens avenues for therapeutic interventions aimed at extending reproductive longevity and optimizing outcomes in assisted reproductive technologies.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.