Evidence map›Paper›PMID 40736675›Full record

ArticleCellular and molecular life sciences : CMLS2025

Proteome of oocyte spindle identifies Ccdc69 regulates spindle assembly like "band-tightening spell".

Jia-Ni Guo, Liu Zhu, Tie-Gang Meng, Yi-Na Zhang, Si-Min Sun, Xue-Mei Yang, Bing-Wang Zhao, Yi-Ke Lu, Yuan-Hong Xu, Wei Yue and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Jia-Ni Guo *State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, #Beichen West Rd, Chaoyang, Beijing, 100101, China.
Liu Zhu *Department of Medical Research Center, State Key Laboratory for Complex, Severe and Rare Diseases, Clinical Research Institute, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China.
Tie-Gang MengGuangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hong Kong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China.
Yi-Na ZhangState Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, #Beichen West Rd, Chaoyang, Beijing, 100101, China.
Si-Min SunState Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, #Beichen West Rd, Chaoyang, Beijing, 100101, China.
Xue-Mei YangState Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, #Beichen West Rd, Chaoyang, Beijing, 100101, China.
Bing-Wang ZhaoState Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, #Beichen West Rd, Chaoyang, Beijing, 100101, China.
Yi-Ke LuState Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, #Beichen West Rd, Chaoyang, Beijing, 100101, China.
Yuan-Hong XuState Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, #Beichen West Rd, Chaoyang, Beijing, 100101, China.
Wei YueState Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, #Beichen West Rd, Chaoyang, Beijing, 100101, China.
Zhiming HanState Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, #Beichen West Rd, Chaoyang, Beijing, 100101, China. hanzm@ioz.ac.cn.
Catherine C L WongDepartment of Medical Research Center, State Key Laboratory for Complex, Severe and Rare Diseases, Clinical Research Institute, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China. catherine_wong@bjmu.edu.cn.
Zhen-Bo WangState Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, #Beichen West Rd, Chaoyang, Beijing, 100101, China. wangzb@ioz.ac.cn.ORCID http://orcid.org/0000-0001-5013-251X

Funding

CAMS Innovation Fund for Medical Sciences 2022-12M-1-004Ministry of Science and Technology of China 2022YFA0806000National Key Research and Development Program of China 2022YFC2702201National Natural Science Foundation of China 82171646
6 · The paper itself

Abstract

Meiotic spindle is an intricate structure and required for chromosome segregation and the proper meiotic progression during oocyte maturation, and its function is regulated by a complex network of proteins located at spindle and its peripheral region. However, proteome of meiotic spindle remains poorly characterized. Here, we acquired the proteomic profile of spindles isolated from metaphase I (MI) and metaphase II (MII) mouse oocytes. In particular, we identified Ccdc69 as a novel regulator of spindle assembly in mouse oocytes. Although deletion of Ccdc69 did not affect female fertility, the MI spindles were elongated in Ccdc69 knockout oocytes. Overexpression of Ccdc69 induced spindle defects by reducing microtubule formation and disturbing acentriolar microtubule organization centers (aMTOCs) distribution. Furthermore, Ccdc69 overexpression impaired kinetochore-microtubule (K-MT) attachment and delayed meiotic progression by abnormal activation of spindle assembly checkpoint (SAC). Taken together, our study depicts the proteome of spindles during mouse oocyte maturation and demonstrates that Ccdc69 regulates spindle assembly and meiotic progression the way similar to "The Tightening Spell of Sun Wukong's Golden Headband" in the famous Chinese Classic Journey to the West.

Indexed as

OocytesProteomeSpindle ApparatusAnimalsFemaleKinetochoresMeiosisMetaphaseMiceMice, KnockoutMicrotubulesM Phase Cell Cycle CheckpointsProteomicsProteomeCcdc69MeiosisOocyteProteomeSpindle assembly

Identifiers

PMID40736675
PMCPMC12311101

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.