ArticleMethods in molecular biology (Clifton, N.J.)2025
Optical Coherence Microscopy for Oocyte Imaging.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Optical coherence microscopy (OCM) is a novel approach to fluorophore-free 3D live imaging of cells, particularly mammalian oocytes and embryos. It allows for 3D high-resolution visualization of the intracellular architecture: nuclei with nucleoli, metaphase spindles, and networks of membranous structures. Moreover, as it is compatible with time-lapse imaging, it enables monitoring and quantitative analysis of the dynamic behavior of these organelles over time. Importantly, OCM, when imaging settings are properly optimized, is safe for oocytes and embryos and does not negatively affect their developmental capabilities. Therefore, OCM is an interesting alternative to currently used imaging techniques, not only in basic research but also in clinical applications. In the present chapter, we describe the main principles of spectral OCM and show how this technique can be applied to visualize mouse oocytes.
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