ReviewMethods in molecular biology (Clifton, N.J.)2025
Current Progress in Phased Genome Assembly from Long-Read DNA Sequencing Data.
Review in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Progress in Flax Genome Assembly from Nanopore Sequencing Data.Plants (Basel, Switzerland) · 2026Article
- The effect of human-specific genetic variants on neuronal spinogenesis.Frontiers in genetics · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome assembly is a core task in the field of genomics. The availability of long-read sequencing technologies enabled the construction of high-quality complex genomes, including phasing of heterozygous contigs. This chapter provides an overview of the main algorithmic techniques for genome assembly. It starts revisiting the two main data structures used for unphased genome assembly, namely, the overlap graph and the de Bruijn graph. Then, it describes current protocols and sequencing alternatives such as trio data and Hi-C to provide long-range information for scaffolding and phasing. Next, it describes the metrics that have been developed to evaluate completeness accuracy and base pair quality. Finally, this chapter provides detailed information on the core algorithmic ideas of four different tools to perform phased genome assemblies (FALCON, HiCanu, Hifiasm, and NGSEP). A review of classic techniques for phasing based on aligned reads is included to provide a context of basic concepts needed to understand the algorithms implemented in the genome assemblers.
Indexed as
Identifiers
40736893What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.