Evidence mapPaperPMID 40739397Full record

ArticleScientific reports2025

Targeting the proliferation of glioblastoma cells and enhancement of doxorubicin and temozolomide cytotoxicity through inhibition of PFKFB4 and HMOX1 genes with siRNAs.

Hamzeh J Al-Ameer, Malek Zihlif, Ahmed Maslat, Wajdy J Al-Awaida, Amani Marwan Ayyash, Amer Imraish, Nidal Al-Qinna, Tareq Al-Omari, Talal Al-Qaisi, Walid Al-Zyoud and 6 more

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Hamzeh J Al-AmeerDepartment of Biotechnology, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman, 19328, Jordan. h.alameer@ammanu.edu.jo.ORCID https://orcid.org/0000-0002-1681-6747
Malek ZihlifDepartment of Pharmacology, Faculty of Medicine, The University of Jordan, Amman, 11942, Jordan.ORCID https://orcid.org/0000-0002-8005-3908
Ahmed MaslatDepartment of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, 21163, Jordan.ORCID https://orcid.org/0000-0003-3808-2788
Wajdy J Al-AwaidaDepartment of Biology and Biotechnology, Faculty of Science, American University of Madaba, Madaba, 17110, Jordan.ORCID https://orcid.org/0000-0003-3095-2224
Amani Marwan AyyashDepartment of Pharmacy, Faculty of Health Sciences, American University of Madaba, Madaba, 17110, Jordan.ORCID https://orcid.org/0000-0001-8246-1473
Amer ImraishDepartment of Biological Sciences, School of Science, The University of Jordan, Amman, 11942, Jordan.ORCID https://orcid.org/0000-0003-1191-2905
Nidal Al-QinnaPharmaceutical Center (UPPC), Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, 11196, Jordan.ORCID https://orcid.org/0000-0002-9628-6036
Tareq Al-OmariDepartment of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, 21163, Jordan.ORCID https://orcid.org/0000-0002-7604-5489
Talal Al-QaisiDepartment of Biotechnology, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman, 19328, Jordan.ORCID https://orcid.org/0000-0002-3646-657X
Walid Al-ZyoudDepartment of Biomedical Engineering, School of Applied Medical Sciences, German Jordanian University, Amman, 11180, Jordan.ORCID https://orcid.org/0000-0002-3772-5617
Bayan T AlzubiDepartment of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman, 19328, Jordan.
Ali M AtoomDepartment of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman, 19328, Jordan.ORCID https://orcid.org/0000-0001-9742-4253
Isam A FattashDepartment of Biology and Biotechnology, Faculty of Science, American University of Madaba, Madaba, 17110, Jordan.
Shubhankar AmbikeInstitute of Virology, School of Medicine, Technische Universität München / Helmholtz Zentrum München, Trogerstr. 30, 81675, Munich, Germany.ORCID https://orcid.org/0000-0003-1089-459X
Khang Wen GohFaculty of Data Science and Information Technology, INTI International University, Nilai, 71800, Malaysia.
Yulia Sh GushchinaDepartment of General and Clinical Pharmacology, Medical Institute, Peoples' Friendship University of Russia (RUDN University), Moscow, 117198, Russia.

Funding

This study was supported by the deanship of scientific research and graduate studies at Yarmouk University (Research Fund No.79/2021). 79/2021
6 · The paper itself

Abstract

Glioblastoma multiforme continues to be one of the most aggressive brain cancers, posing a serious health challenge, as it offers a median survival of only 15-23 months and a 5-year survival rate of less than 6%. Current treatments often prove inadequate, underscoring the urgency for new therapeutic strategies. This study investigated the potential of silencing the PFKFB4 and HMOX1 genes in U87-MG glioblastoma cells using small interfering RNAs (siRNAs), both alone and alongside the chemotherapeutic agents temozolomide (TMZ) and doxorubicin (DOX). Through MTT assays, qPCR, and wound healing techniques, we assessed cell viability, gene expression, and cell migration. Notably, siPFKFB4 enhanced DOX's cytotoxic effect, reducing its IC

Indexed as

Brain NeoplasmsDoxorubicinGlioblastomaHeme Oxygenase-1Phosphofructokinase-2RNA, Small InterferingApoptosisCell Line, TumorCell MovementCell ProliferationCell SurvivalGene Expression Regulation, NeoplasticHumansTemozolomideDoxorubicinHeme Oxygenase-1HMOX1 protein, humanPFKFB4 protein, humanPhosphofructokinase-2RNA, Small InterferingTemozolomideDoxorubicinGlioblastomaHMOX1Human healthPFKFB4siRNATemozolomide

Identifiers

PMID40739397
PMCPMC12311046

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.