Evidence map›Paper›PMID 40746554›Full record

ArticleFrontiers in immunology2025

Amblyostatin-1, the first salivary cystatin with host immunomodulatory and anti-inflammatory properties from the Neotropical tick

Wilson Santos Molari, Mohamed Amine Jmel, Josiane Betim Assis, Alan Frazão-Silva, Júlia Moura Bernardi, Gretta Huamanrayme, José María Medina, Eliane Esteves, Solange Cristina Antão, Gabriel Cerqueira Alves Costa and 6 more

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In one paragraph

Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Wilson Santos MolariDepartment of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Mohamed Amine JmelInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czechia.
Josiane Betim AssisDepartment of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Alan Frazão-SilvaDepartment of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Júlia Moura BernardiDepartment of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Gretta HuamanraymeDepartment of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
José María MedinaDepartamento de Genética, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
Eliane EstevesDepartment of Microbiology and Immunology, Frederick P. Whiddon College of Medicine, University of South Alabama, Mobile, AL, United States.
Solange Cristina AntãoDepartment of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
Gabriel Cerqueira Alves CostaDepartment of Biochemistry, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.
Aparecida Sadae TanakaDepartment of Biochemistry, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.
Andréa Cristina FogaçaDepartment of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
Zdenek FrantaDepartment of Chemistry, Faculty of Science, University of South Bohemia, České Budějovice, Czechia.
Lucas TirloniTick-Pathogen Transmission Unit, Laboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Hamilton, MT, United States.
Michalis KotsyfakisInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czechia.
Anderson Sá-NunesDepartment of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: The Neotropical tick Methods: Bioinformatic tools were employed for Results: Based on its sequence and predicted three-dimensional structure, Amblyostatin-1 is classified as an I25B cystatin, and its recombinant form selectively inhibits cathepsins L, C, and S at different rates, with a low nanomolar Discussion: These findings establish Amblyostatin-1 as a novel salivary cystatin with immunomodulatory and anti-inflammatory properties, highlighting its potential as an immunobiological agent.

Indexed as

AmblyommaAnti-Inflammatory AgentsArachnid VectorsArthropod ProteinsCystatinsSalivary CystatinsAnimalsDendritic CellsFemaleMiceAnti-Inflammatory AgentsArthropod ProteinsCystatinsSalivary CystatinsAmblyomma sculptumAmblyostatin-1immunomodulationinflammationtick-host interactiontick saliva

Identifiers

PMID40746554
PMCPMC12310653

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.