Evidence mapPaperPMID 40748430Full record

ArticleMolecular neurobiology2025

Semaglutide Mitigates Ischemic Brain Injury by Inhibiting Ferroptosis via Modulation of FoXO1 and DRP1 Pathways.

Weihua Wang, Meng Pang, Yifeng Zhang, Shuai Hou, Yulei Xia, Youkui Shi, Xiaojun Zhang, Yanqiang Wang

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Molecular neurobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Weihua WangEmergency Department, Affiliated Hospital of Shandong Second Medical University, School of Clinical Medicine, Shandong Second Medical University, No 2428 YuHe Road, KuiWen, Weifang, 261041, Shandong, China.
Meng PangDepartment of Neurology II, Affiliated Hospital of Shandong Second Medical University, School of Clinical Medicine, Shandong Second Medical University, No 2428 YuHe Road, KuiWen, Weifang, 261041, Shandong, China.
Yifeng ZhangDepartment of Neurology II, Affiliated Hospital of Shandong Second Medical University, School of Clinical Medicine, Shandong Second Medical University, No 2428 YuHe Road, KuiWen, Weifang, 261041, Shandong, China.
Shuai HouEmergency Department, Affiliated Hospital of Shandong Second Medical University, School of Clinical Medicine, Shandong Second Medical University, No 2428 YuHe Road, KuiWen, Weifang, 261041, Shandong, China.
Yulei XiaDepartment of Neurology II, Affiliated Hospital of Shandong Second Medical University, School of Clinical Medicine, Shandong Second Medical University, No 2428 YuHe Road, KuiWen, Weifang, 261041, Shandong, China.
Youkui ShiEmergency Department, Affiliated Hospital of Shandong Second Medical University, School of Clinical Medicine, Shandong Second Medical University, No 2428 YuHe Road, KuiWen, Weifang, 261041, Shandong, China.
Xiaojun ZhangDepartment of Neurology II, Affiliated Hospital of Shandong Second Medical University, School of Clinical Medicine, Shandong Second Medical University, No 2428 YuHe Road, KuiWen, Weifang, 261041, Shandong, China.
Yanqiang WangDepartment of Neurology II, Affiliated Hospital of Shandong Second Medical University, School of Clinical Medicine, Shandong Second Medical University, No 2428 YuHe Road, KuiWen, Weifang, 261041, Shandong, China. wangyq6@mail3.sysu.edu.cn.

Funding

Health China•BuChang ZhiYuan Public welfare projects for heart and brain health under Grant No.HIGHER2023072Shandong Second Medical University Affiliated Hospital Technology Development Project 2023FYM006Yuan Du Scholars, National Natural Science Foundation of China 81870943
6 · The paper itself

Abstract

Multiple pathogenic processes contribute to cerebral ischemia-reperfusion injury (CIRI); however, their relative importance and sequence remain unclear, warranting further investigation. Although our previous studies showed that inhibiting ferroptosis mitigates brain damage after ischemic stroke, the crosstalk among intracellular pathways remains poorly understood. The protective effects of semaglutide, a novel glucagon-like peptide-1 receptor agonist (GLP-1RA), in ischemic stroke have yet to be fully elucidated. In this study, bioinformatics analysis was conducted to predict the potential therapeutic mechanisms of semaglutide in CIRI, highlighting the involvement of the FoXO1-autophagy pathway and mitochondrial dynamics. A rat's middle cerebral artery occlusion/reperfusion (MCAO/R) model was established to validate these predictions. Histopathological examination demonstrated that semaglutide alleviated pathological damage and reduced neuronal apoptosis. Using Western blotting, RT-PCR, immunofluorescence, and ELISA, we assessed key markers-GLP-1R, FoXO1, Beclin1, DRP1, Mfn2, ATP, and ROS-in the ischemic penumbra. The results showed that semaglutide inhibited autophagy (Beclin1), suppressed mitochondrial fission (DRP1), promoted mitochondrial fusion (Mfn2), and improved mitochondrial function, as reflected by increased ATP production and reduced ROS levels. Furthermore, we investigated the involvement of FoXO1 and mitochondrial function in the regulation of ferroptosis. The findings suggest that semaglutide inhibits ferroptosis by activating GLP-1R, thereby modulating the intracellular FoXO1/GPX4 and DRP1/ACSL4 signaling pathways. In summary, semaglutide acts through GLP-1R to regulate FoXO1-mediated autophagy, mitochondrial dynamics, and ferroptosis, as well as to modulate DRP1-dependent mitochondrial fission and ferroptosis. Together, these mechanisms account for its protective effects against ischemic stroke.

Indexed as

Brain IschemiaDynaminsFerroptosisForkhead Box Protein O1Glucagon-Like PeptidesSignal TransductionAnimalsAutophagyGlucagon-Like Peptide 1Infarction, Middle Cerebral ArteryMaleMitochondriaMitochondrial DynamicsRatsRats, Sprague-DawleyReperfusion InjuryDnm1l protein, ratDynaminsForkhead Box Protein O1Foxo1 protein, ratGlucagon-Like Peptide 1Glucagon-Like PeptidesSemaglutideCerebral ischemia/reperfusion injuryDRP1FerroptosisFoXO1Mitochondrial dynamics

Identifiers

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.