Evidence map›Paper›PMID 40754775›Full record

ArticleBMB reports2025

Ubiquitin specific protease 7 deubiquitinates and regulates Aurora B-mediated cytokinesis.

Kamini Kaushal, Ainsley Mike Antao, Soumyadip Das, Sammy L Kim, Girish Birappa, Sripriya Rajkumar, D A Ayush Gowda, C Bindu Ajaykumar, Vijai Singh, Keesung Kim and 2 more

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Article in BMB reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Kamini KaushalGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea.
Ainsley Mike AntaoGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea.
Soumyadip DasGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea.
Sammy L KimDepartment of Biological Sciences, College of Sang-Huh Life Science, Konkuk University, Seoul 05029, Korea.
Girish BirappaGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea.
Sripriya RajkumarGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea.
D A Ayush GowdaGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea.
C Bindu AjaykumarGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea.
Vijai SinghDepartment of Biosciences, School of Science, Indrashil University, Gujarat, Mehsana 382715, India.
Keesung KimResearch Institute of Advanced Materials, College of Engineering, Seoul National University, Seoul 08826, Korea.
Bharathi SureshGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea.
Suresh RamakrishnaGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea; College of Medicine, Hanyang University, Seoul 04763, Korea.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aurora B is a widely studied mitotic checkpoint kinase that forms a part of the chromosomal passenger complex. The entry to and exit from mitosis are exquisitely controlled by Aurora B proteins, which regulate mitotic phases including chromosomal condensation, segregation, and cytokinesis, ensuring faithful propagation of daughter cells. Abnormal regulation of Aurora B proteins during the cell cycle can cause increased chromosomal segregation errors and ultimately lead to cancer. Thus, it is important to understand the key mechanisms that can modulate Aurora B protein levels during the cell cycle. Therefore, in this study we demonstrated the role of Ubiquitin-specific protease 7 (USP7) in regulating Aurora B protein level. Aurora B protein levels are upregulated when USP7 is dose-dependently increased, and downregulated when USP7 is depleted. By co-immunoprecipitation and Duolink assays, we demonstrated that USP7 interact with Aurora B. Furthermore, by treating cycloheximide we showed that USP7 extends the Aurora B protein half-life by its deubiquitinating activity. Finally, CRISPR/Cas9-mediated USP7 knockout produces severe nuclear structural defects causing multi-nucleation and cytokinesis failures, suggesting that the important role of USP7 during mitotic progression in stabilizing Aurora B. [BMB Reports 2025; 58(8): 350-356].

Indexed as

Aurora Kinase BCytokinesisUbiquitin-Specific Peptidase 7HEK293 CellsHeLa CellsHumansMitosisUbiquitinationAURKB protein, humanAurora Kinase BUbiquitin-Specific Peptidase 7USP7 protein, human

Identifiers

PMID40754775
PMCPMC12402693

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.