ArticleMethodsX2025
Simplified method for purifying full-length major type IV pilins: PilA from
Article in MethodsX, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Expression of soluble Type IV Minor Pilins and isolation of abioRxiv : the preprint server for biology · 2026Article
- A Paradigm Shift in Microbial Protein Manufacturing.Life (Basel, Switzerland) · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type IV major pilins are membrane-associated proteins that play critical roles in bacterial adaptation and survival across diverse environmental conditions, including potentially extreme ones. However, their expression and purification remain challenging due to their hydrophobic nature and tendency to aggregate. Here, we present a cost-effective method for the production and purification of recombinant full-length PilA, the major type IV pilin from the acidophilic bacterium
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.