ArticleAlcohol, clinical & experimental research2025
High-throughput quantitation of acetaldehyde and ethanol in mice using gas chromatography/mass spectrometry positive chemical ionization.
Article in Alcohol, clinical & experimental research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The trial behind it
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Who cites it
3 citing papers in PubMed.
- Timeless and Stainless Alcohol: Concentric Waves from Its Oxidative Metabolism and Related Oxidative Stress.Antioxidants (Basel, Switzerland) · 2026Review
- Hepatic Aquaporin 8 Promotes Alcohol Consumption and Ameliorates Alcohol-Induced Liver Injury by Facilitating Acetaldehyde Excretion.International journal of biological sciences · 2026Article
- High-throughput quantitation of acetaldehyde and ethanol in mice using gas chromatography/mass spectrometry positive chemical ionization.Alcohol, clinical & experimental research · 2025Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
backgroundAcetaldehyde, an immediate ethanol metabolite, mediates many ethanol-induced behavioral effects and is both psychoactive and toxic to animals and humans. Monitoring the kinetics of acetaldehyde using rodent models of alcohol misuse is essential for understanding and managing ethanol-associated diseases. However, quantitation of acetaldehyde in biological specimens after alcohol consumption has been challenging due to its high volatility, relatively low concentrations, and strong reactivity toward biochemical molecules. It was necessary to develop and establish an accurate and high-throughput method to quantitate acetaldehyde and ethanol.
methodsGas chromatography/mass spectrometry in positive chemical ionization mode coupled with a 111-vial headspace autosampler was employed to quantitate acetaldehyde and ethanol using
resultsThe method was validated and applied to quantitate acetaldehyde and ethanol in blood and tissues from multiple mouse studies on ethanol metabolism. Acetaldehyde and ethanol were well-resolved from chromatographic interferences with linear ranges of 6.25-800 μM for acetaldehyde and 1.25-160 mM for ethanol. Both regression coefficients for calibration curves were >0.999. The within- and between-run precisions for ethanol in plasma, whole blood, and serum were all <5.0%, and for acetaldehyde in plasma and serum were <9.0%, while in whole blood it was 19.2%. Sample throughput was on the order of 60 samples per 15 h daily, with a maximum of 111 per batch.
conclusionsDespite some limitations, this validated method proved to be specific, accurate, and reproducible for high-throughput quantitation of acetaldehyde and ethanol in rodent plasma, whole blood, serum, and visceral organs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.