Evidence mapPaperPMID 40761383Full record

ArticleJournal of inflammation research2025

Astragaloside IV Attenuates Chronic Prostatitis by Activating Keap1/Nrf2/HO-1 Pathway: Suppressing Ferroptosis and Enhancing Antioxidant Defense.

Jintao Shi, Yang Xuan, Qinyu Zhang, Jiangtao Chen, Weihan Zhu, Hao Zhang, Yue Duan

Abstract read
In one paragraph

Article in Journal of inflammation research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jintao Shi *The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, People's Republic of China.ORCID 0009-0009-3553-5535
Yang Xuan *The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, People's Republic of China.
Qinyu Zhang *The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, People's Republic of China.ORCID 0009-0007-9317-6820
Jiangtao ChenThe Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, People's Republic of China.
Weihan ZhuThe Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, People's Republic of China.
Hao ZhangDepartment of Urology and Andrology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310005, People's Republic of China.
Yue DuanDepartment of Urology and Andrology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310005, People's Republic of China.ORCID 0000-0002-4479-4231

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), a chronic inflammatory disorder with complex etiology and limited treatment options, is closely associated with oxidative stress and regulates cell death. Ferroptosis-an iron-dependent cell death driven by lipid peroxidation-amplifies CP/CPPS inflammation by concurrently triggering mitochondrial apoptosis and NLRP3 inflammasome activation, while Keap1/Nrf2/HO-1 axis serves as a central regulator bridging ferroptotic, apoptotic, and inflammatory cell death pathways. Astragaloside IV (AS-IV), a primary bioactive component of Astragalus membranaceus with established clinical use in urological therapies and favorable pharmacokinetics, was prioritized over structural analogs due to its unique dual-phase modulation: enhancing Nrf2 nuclear translocation without suppressing NF-κB-mediated immune surveillance. However, regulatory mechanisms linking AS-IV to ferroptosis inhibition in CP/CPPS remain unknown. Patients and Methods: This study aimed to investigate the therapeutic potential of astragaloside IV (the primary bioactive component of Results: AS-IV ameliorated prostatic tissue inflammation and fibrosis, reduced lipid peroxidation marker malondialdehyde (MDA) levels, and enhanced antioxidant indicators, including glutathione (GSH) content and glutathione peroxidase 4 (GPX4) activity. Western blotting and immunohistochemical analyses further confirmed that AS-IV activated the antioxidant pathway by suppressing Keap1 expression, promoting Nrf2 nuclear translocation, and upregulating heme oxygenase-1 (HO-1) protein levels. Concurrently, pro-inflammatory cytokine levels, including tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), were markedly reduced. Conclusion: This is the first study to demonstrate that AS-IV alleviates type III CP pathological damage by inhibiting ferroptosis via the Keap1/Nrf2/HO-1 axis, thereby providing experimental evidence for the development of multi-target therapeutic strategies based on natural products.

Indexed as

cellular redox balancelipid peroxidationNrf2oxidative stresspharmacological activationprostatic inflammation

Identifiers

PMID40761383
PMCPMC12318839

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.