Evidence map›Paper›PMID 40764919›Full record

ArticleBMC microbiology2025

Integrated transcriptomic and metabolomic analysis of the antibacterial mechanism of Rhizoma Coptidis extract against Staphylococcus epidermidis ATCC 35984.

Lizhuang Wang, Yan Xing, Shuai Yang, Huan Zhang, Laiji Ma, Li Shao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Lizhuang Wang *School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, 201418, P. R. China.
Yan Xing *School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, 201418, P. R. China.
Shuai YangShandong Academy of Pharmaceutical Sciences, Jinan, Shandong, 250098, P. R. China.
Huan ZhangSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, 201418, P. R. China.
Laiji MaSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, 201418, P. R. China.
Li ShaoSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, 201418, P. R. China. teamo-lily@163.com.

Funding

Collaborative Innovation Center of Fragrance Flavour and Cosmetics 1021ZK230016013
6 · The paper itself

Abstract

backgroundBiofilm formation is a key virulence factor in Staphylococcus epidermidis. Rhizoma Coptidis, the medicinal root of Coptis chinensis, has been traditionally used in Chinese medicine for its broad-spectrum antimicrobial properties.

resultsThis study investigated the antibacterial and anti-biofilm effects of Rhizoma Coptidis extract against S. epidermidis ATCC 35984. UPLC-MS/MS analysis revealed the chemical composition of the extract. The extract significantly reduced ATPase and succinate dehydrogenase activities, decreased membrane potential, and disrupted cell wall and membrane integrity. These effects led to increased extracellular alkaline phosphatase activity and leakage of proteins and nucleic acids. Anti-biofilm activity was further confirmed using scanning electron microscopy and confocal laser scanning microscopy. Transcriptomic and metabolomic analyses identified significant changes in 366 genes and 286 metabolites after treatment. Integrated omics analysis suggested that the extract impaired cell membrane and wall integrity, disrupted amino acid and nucleic acid metabolism, inhibited the TCA cycle, reduced nitrate reductase activity, suppressed efflux systems, and hindered biofilm formation.

conclusionsThe study provides new insights into the antibacterial and anti-biofilm mechanisms of Rhizoma Coptidis extract against S. epidermidis, highlighting its potential as a therapeutic agent for combating biofilm-associated infections.

Indexed as

Anti-Bacterial AgentsCoptisDrugs, Chinese HerbalPlant ExtractsStaphylococcus epidermidisBacterial ProteinsBiofilmsCell MembraneCell WallCoptis chinensisGene Expression ProfilingMetabolomeMetabolomicsMicrobial Sensitivity TestsTandem Mass SpectrometryTranscriptomeAnti-Bacterial AgentsBacterial ProteinsCoptidis rhizoma extractDrugs, Chinese HerbalPlant ExtractsAntibacterial activityBiofilmMetabolomicRhizoma Coptidis extractStaphylococcus epidermidisTranscriptomic

Identifiers

PMID40764919
PMCPMC12323277

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.