Evidence map›Paper›PMID 40765420›Full record

ArticleGlia2025

AAV-Based Intracerebral Administration of BDNF Promotes Myelin Repair and Cognitive Improvement After Cuprizone-Induced Demyelination.

Yousra El Ouaamari, Leonardo Ricciardi, Sanne van der Heijden, Antonia Peter, Jorrit De Waele, Jasper Van den Bos, Debby Van Dam, Elke Calus, Sarah Kuhn, Waleed Marei and 5 more

Abstract read
In one paragraph

Article in Glia, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yousra El OuaamariLaboratory of Experimental Haematology, Vaccine and Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.ORCID 0000-0003-4075-2445
Leonardo RicciardiLaboratory of Experimental Haematology, Vaccine and Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
Sanne van der HeijdenCenter for Oncology Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, Antwerp, Belgium.
Antonia PeterLaboratory of Experimental Haematology, Vaccine and Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
Jorrit De WaeleCenter for Oncology Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, Antwerp, Belgium.ORCID 0000-0002-9999-6156
Jasper Van den BosLaboratory of Experimental Haematology, Vaccine and Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.ORCID 0000-0002-6011-0960
Debby Van DamNeurochemistry & Behaviour Group, Experimental Neurobiology Unit (ENU), Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium.
Elke CalusNeurochemistry & Behaviour Group, Experimental Neurobiology Unit (ENU), Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium.
Sarah KuhnWellcome Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Northern Ireland, UK.
Waleed MareiGamete Research Centre, Department of Veterinary Sciences, University of Antwerp, Antwerp, Belgium.
Yvonne DombrowskiWellcome Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Northern Ireland, UK.
Marleen VerhoyeμNEURO Research Centre of Excellence, University of Antwerp, Antwerp, Belgium.
Peter PonsaertsLaboratory of Experimental Haematology, Vaccine and Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.ORCID 0000-0002-1892-6499
Inez WensLaboratory of Experimental Haematology, Vaccine and Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
Nathalie CoolsLaboratory of Experimental Haematology, Vaccine and Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.

Funding

Charcot FoundationEuropean Union's Horizon 2020 Research and Innovation Programme Marie Skłodowska-Curie grant 813263European Union's Horizon 2020 Research and Innovation Programme Marie Skłodowska-Curie grant 860003Fritz-Thyssen FoundationFWO I000123NFWO I003420NRoche BelgiumThe Leverhulme TrustUniversity of Antwerp BOF 42551University of Antwerp BOF 49061University of Antwerp BOF Methusalem
6 · The paper itself

Abstract

Multiple sclerosis (MS) is a chronic neurological disorder involving immune-mediated demyelination and neurodegeneration in the central nervous system (CNS). Current therapies primarily target inflammation, with limited strategies to promote remyelination or neural repair. This study explores the therapeutic potential of Brain-Derived Neurotrophic Factor (BDNF) delivered via an adeno-associated virus (AAV) vector to enhance remyelination and improve cognitive function in a subchronic cuprizone (CPZ)-induced demyelination mouse model. Sixty female C57BL/6 mice were used, with half receiving a 7-week CPZ diet to induce oligodendrocyte loss. After demyelination, mice were treated with AAV-BDNF, AAV-eGFP, or saline injections into the corpus callosum (CC), followed by a 5-week recovery phase. Behavioral assessments revealed improved cognitive performance with BDNF treatment, demonstrated by increased latency in passive avoidance tests. Immunofluorescence analysis showed increased proliferation and maturation of oligodendrocyte progenitor cells, with higher PDGFRα and CC1 markers, alongside elevated MBP. Transmission electron microscopy (TEM) indicated thicker myelin sheaths and a higher percentage of myelinated axons in AAV-BDNF-treated mice. Mitochondrial analyses revealed that BDNF treatment preserved mitochondrial integrity, with reduced swelling and improved structural regularity. Inflammatory markers showed no differences in Iba1 but indicated a trend of reduced astrocytic activation with BDNF. These results demonstrate that AAV-BDNF therapy enhances remyelination, myelin integrity, mitochondrial structure, and cognitive function in a CPZ model, underscoring its potential for treating MS. BDNF-based strategies may offer innovative avenues to improve neurological recovery and address unmet needs in MS management.

Indexed as

Brain-Derived Neurotrophic FactorDemyelinating DiseasesMyelin SheathAnimalsCognitionCorpus CallosumCuprizoneDependovirusDisease Models, AnimalFemaleMiceMice, Inbred C57BLOligodendrogliaRemyelinationBdnf protein, mouseBrain-Derived Neurotrophic FactorCuprizonebrain‐derived neurotrophic factor (BDNF)cognitive functionmultiple sclerosis (MS)oligodendrocytesremyelination

Identifiers

PMID40765420
PMCPMC12436986

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.