Evidence map›Paper›PMID 40768435›Full record

ArticlePloS one2025

Development of a tissue-specific bioscaffold for intestinal stem cell culture.

Sachin Kakar, Mathieu F Derouet, Liyue Zhang, Connor J Gillis, Frederikke Larsen, Arghya Paul, Lauren E Flynn, Samuel Asfaha

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sachin KakarDepartment of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada.
Mathieu F DerouetDepartment of Medicine, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada.
Liyue ZhangDepartment of Medicine, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada.
Connor J GillisSchool of Biomedical Engineering, Faculty of Engineering, The University of Western Ontario, London, Ontario, Canada.
Frederikke LarsenDepartment of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada.
Arghya PaulDepartment of Chemical and Biochemical Engineering, Faculty of Engineering, The University of Western Ontario, London, Ontario, Canada.
Lauren E FlynnDepartment of Chemical and Biochemical Engineering, Faculty of Engineering, The University of Western Ontario, London, Ontario, Canada.
Samuel AsfahaDepartment of Medicine, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada.ORCID https://orcid.org/0000-0001-6972-0338

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The generation of a tissue-specific intestinal hydrogel derived from the native intestine has the potential to support and promote the growth of intestinal organoids. In this study, we aimed to develop hydrogels derived exclusively from intestinal extracellular matrix (ECM) or composites comprised of intestinal ECM combined with alginate that allow for greater tuning of the hydrogel properties. A novel mouse intestinal decellularization protocol was developed and the ECM characterized. Our analyses demonstrate that our protocol effectively removed cellular and nuclear content while preserving key ECM components including collagens, glycosaminoglycans, fibronectin and laminin. When the decellularized small intestine (DSI) was used to generate hydrogels, the resulting ECM showed bioactivity as demonstrated by metabolic and pro-proliferative effects on NIH 3T3 murine fibroblasts. Importantly, our novel DSI hydrogels also supported murine intestinal and colonic organoid growth similar to Matrigel® controls. These studies demonstrate that murine tissue-specific DSI hydrogels can provide a supportive environment for the culture of intestinal and colonic organoids in vitro.

Indexed as

Cell Culture TechniquesIntestinesIntestine, SmallOrganoidsStem CellsTissue ScaffoldsAlginatesAnimalsCollagenDecellularized Extracellular MatrixExtracellular MatrixHydrogelsMiceNIH 3T3 CellsTissue EngineeringAlginatesCollagenDecellularized Extracellular MatrixHydrogels

Identifiers

PMID40768435
PMCPMC12327626

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.