ArticleBiotechnology journal2025
Unveiling Small Non-Coding RNA Dynamics During Recombinant Adeno-Associated Virus Production.
Article in Biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Expanding Transposase Technology to Regulatory Noncoding RNAs for Stable Expression in CHO Cells.Biotechnology journal · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinant adeno-associated viruses (rAAVs) play a pivotal role in gene therapy, yet the molecular interactions underlying rAAV production in host cells remain incompletely understood. Non-coding RNAs (ncRNAs), particularly microRNAs (miRNAs) and small nucleolar RNAs (snoRNAs), are increasingly recognized as key regulators of viral and cellular processes. This study investigates the dynamic expression profiles of miRNAs and snoRNAs during rAAV plasmid transfection and vector production in HEK293F cells. A total of 142 miRNAs were differentially expressed during the peak phase of rAAV production, with 128 associated with the Gene Ontology term "viral process", indicating broad involvement in host-virus interactions. Target gene analysis linked these miRNAs to biological pathways such as nucleocytoplasmic transport, innate immunity, apoptosis, and transcriptional regulation, highlighting potential roles of miRNAs in shaping the cellular environment during viral vector assembly. In contrast, snoRNAs exhibited more modest changes in expression, yet five were significantly differentially expressed during active production, suggesting a possible, underexplored involvement in viral replication. These findings illuminate the underexplored contributions of ncRNAs to the host response during rAAV biogenesis and provide a valuable resource for understanding how cellular regulatory networks are engaged throughout vector production.
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Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.