ArticlePlant cell reports2025
Reduced stomatal density improves water-use efficiency in grapevine under climate scenarios of decreased water availability.
Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- High-resolution microCT reveals relationships between stomata and interior leaf anatomy in sorghum.Plant physiology · 2026Article
- Transforming viticulture through genomic innovation and integrated pest management for sustainable grape production.NPJ science of plants · 2026Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
key messageThe grapevine VviEPFL9-2 paralog is specifically expressed during leaf expansion and its knockout provide a phenotype with superior adaptation to environmental stresses via reduced stomatal density. In Arabidopsis stomatal initiation relies on the transcription factor SPEECHLESS, which is positively regulated by AtEPFL9, a peptide of the epidermal patterning factor family. In grapevine, two EPFL9 paralogs exist but despite a structural similarity, their specific function remains unclear. In this study, we investigated their distinct functional roles and the extent to which reduced stomatal density (SD) may be beneficial for grapevine in terms of water use. We combined expression analysis of the two paralogs in untreated and ABA-treated leaves with the functional characterization of the two genes using grapevine epfl9-1 and epfl9-2 mutants. A physiological analysis of epfl9-2 mutants under different environmental conditions was also performed. We showed that VviEPFL9-1 is exclusively expressed in leaf primordia, whereas VviEPFL9-2 plays a predominant role in fine-tuning SD during the leaf expansion. An epfl9-2 mutant line with 84% lower SD than wild type, exhibited a significant improvement in intrinsic water-use efficiency under both well-watered and water-stressed conditions, with little trade-off in photosynthesis. When the reduction in SD was close to 60%, photosynthetic rate and stomatal conductance were comparable to WT. Our results provide compelling evidence that VviEPFL9-2 knockout determines a significant reduction in stomatal density without a major impact on photosynthesis which may help optimize the adverse impacts of climate change on viticulture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.