Evidence map›Paper›PMID 40776765›Full record

ArticleACS infectious diseases2025

An Rcs Stress-Based High-Throughput Screen Reveals Novel Gyrase Inhibitors as Indirect Inducers of Cell Envelope Stress in Gram-Negative Bacteria.

Laurence Cleenewerk, Alexandra Otto, Willemijn Wouters, Joost Willemse, Meiling Gao, Vladyslav Lysenko, Jeroen M Punt, Mario van der Stelt, Nathaniel I Martin, Peter van Ulsen and 1 more

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Article in ACS infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Laurence CleenewerkDepartment of Molecular Microbiology, A-LIFE, AIMMS, VU Amsterdam, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands.ORCID 0000-0003-3538-0288
Alexandra OttoDepartment of Molecular Microbiology, A-LIFE, AIMMS, VU Amsterdam, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands.
Willemijn WoutersPivot Park Screening Centre, Kloosterstraat 9, 5349 AB Oss, The Netherlands.
Joost WillemseInstitute of Biology, Microscopy Unit, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands.
Meiling GaoBiological Chemistry Group, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands.
Vladyslav LysenkoBiological Chemistry Group, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands.ORCID 0000-0001-9236-5821
Jeroen M PuntDepartment of Molecular Physiology, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.ORCID 0000-0002-0887-3557
Mario van der SteltDepartment of Molecular Physiology, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.ORCID 0000-0002-1029-5717
Nathaniel I MartinBiological Chemistry Group, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands.ORCID 0000-0001-8246-3006
Peter van UlsenDepartment of Molecular Microbiology, A-LIFE, AIMMS, VU Amsterdam, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands.
Joen LuirinkDepartment of Molecular Microbiology, A-LIFE, AIMMS, VU Amsterdam, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The highly impermeable cell envelope of Gram-negative bacteria is an important hurdle to the development of novel antibacterials. However, compounds that disrupt the integrity of the cell envelope can act as potent antibiotics by directly inhibiting cell growth and viability or by enhancing the penetration of other, larger antibiotics otherwise unable to pass this barrier. To identify such novel compounds, we used the European Lead Factory compound libraries to screen >500,000 small molecules for inducing the Rcs cell envelope stress response in

Indexed as

Anti-Bacterial AgentsCell MembraneCell WallEscherichia coliGram-Negative BacteriaStress, PhysiologicalTopoisomerase II InhibitorsDNA GyraseHigh-Throughput Screening AssaysMicrobial Sensitivity TestsQuinolonesSOS Response, GeneticsAnti-Bacterial AgentsDNA GyraseQuinolonesTopoisomerase II Inhibitorsantibioticscell envelope stressgyrasehigh-throughput screenquinolones

Identifiers

PMID40776765
PMCPMC12442099

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.