ArticleClinical rheumatology2025
Fire needling acupuncture attenuates synovial inflammation and cartilage degeneration in knee osteoarthritis via SDF-1/CXCR4-mediated macrophage polarization.
Article in Clinical rheumatology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- Adipose-derived mesenchymal stem cell injection into the KI10 acupoint mitigates cartilage damage in KOA rats through PGE2-mediated α7nAChR/NF-κB pathway.Animal models and experimental medicine · 2026Article
- [Advances in immunomodulatory strategies for meniscal regeneration].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2026Review
- Fire needling as a neuromodulatory interface in osteoarthritis management.Clinical rheumatology · 2026Article
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
objectivesThis study aims to investigate the molecular mechanisms underlying the therapeutic effects of fire needling acupuncture (FNA) in alleviating knee osteoarthritis (KOA) symptoms, focusing on the SDF-1/CXCR4 signaling pathway and macrophage polarization.
methodsA KOA mouse model was established using anterior cruciate ligament transection and destabilization of the medial meniscus (ACLT + DMM) surgery. After four weeks, FNA was administered twice weekly for another four weeks. Pain behavior was assessed via weight-bearing and mechanical withdrawal thresholds. Micro-CT, histological staining, immunohistochemistry, and immunofluorescence were utilized to evaluate cartilage and subchondral bone changes, along with relevant markers. Synovial inflammation and macrophage polarization were analyzed, specifically targeting the SDF-1/CXCR4 pathway. The effects of CXCR4 modulation were assessed using AMD3100 (antagonist) and ATI-2341 (agonist).
resultsFNA treatment improved joint function, reduced pain, alleviated synovial inflammation, and mitigated subchondral bone changes. It decreased cartilage damage while upregulating COL2 and SOX9, and downregulating MMP13. The SDF-1/CXCR4 pathway was activated, leading to a shift in macrophage polarization characterized by reduced pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) and increased anti-inflammatory cytokine IL-10. M0 macrophage marker F4/80 and M1 marker CD86 were downregulated, whereas M2 marker CD206 was upregulated. The ATI-2341 group exhibited therapeutic effects similar to FNA, while the AMD3100 and AMD3100 + FNA groups showed reduced efficacy.
conclusionsFNA alleviates KOA by activating the SDF-1/CXCR4 pathway, modulating macrophage polarization, and reducing cartilage damage and inflammation. These findings highlight the therapeutic potential of FNA in the management of KOA. Key Points • Fire needling acupuncture (FNA) differentially regulates SDF-1/CXCR4 signaling in knee osteoarthritis (KOA), where synovial/cartilaginous upregulation enhances mesenchymal stem cell (MSC) homing to potentiate tissue repair, while subchondral suppression mitigates pathological bone remodeling. • FNA activates the JAK1/STAT6/PPAR-γ axis to drive macrophage phenotypic switching from pro-inflammatory M1 to anti-inflammatory M2 states, thereby preserving cartilage matrix integrity through reduced collagenase activity. • Combinatorial CXCR4 inhibition/agonist studies demonstrate FNA's targetable effects across tissue compartments, providing a mechanistic framework to refine acupuncture-based regenerative strategies. • This work establishes a precision medicine framework for degenerative joint diseases by bridging traditional acupuncture with immunomodulatory biologics to target immune-microenvironment crosstalk.
Indexed as
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.