Evidence map›Paper›PMID 40779195›Full record

ArticleMolecular biology reports2025

Probiotic intervention alters immune gene expression and tumor characteristics in experimental breast cancer.

Niloofar Khosrobaki Barchelouei, Mohammad Hossein Yazdi, Setareh Haghighat

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Niloofar Khosrobaki BarcheloueiDepartment of Microbiology, TeMS.C, Islamic Azad University, Tehran, Iran.
Mohammad Hossein YazdiBiotechnology Research Center, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. mh-yazdi@tums.ac.ir.
Setareh HaghighatDepartment of Microbiology, TeMS.C, Islamic Azad University, Tehran, Iran. haghighat.s@iau.ac.ir.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBreast cancer is a prevalent malignancy in women worldwide, with recurrence and treatment-related side effects posing significant challenges. Immunotherapy has shown promise, yet an immunosuppressive tumor microenvironment can hinder its effectiveness. Emerging evidence suggests that probiotics, particularly Lactobacillus species, may positively influence immune responses and tumor progression in the context of cancer therapy.

methodsThis study evaluated the effects of Lactobacillus reuteri PTCC1058 on tumor development and immune responses in a mouse model of breast cancer induced by 4T1 mouse breast carcinoma cells. Female BALB/c mice were divided into probiotic and control groups, receiving oral supplementation of the probiotic or PBS, respectively, for 30 days before and after tumor induction. Tumor volume and survival were assessed over 70 days. The expression levels of key immune mediators, tumor Necrosis Factor alpha (TNF alpha), Interleukin 10 (IL-10), transforming growth factor beta (TGF-β), Indoleamine 2,3-dioxygenase (IDO), and Fas ligand (FasL) were measured using quantitative real-time PCR, and histopathological analysis was conducted on tumor tissues.

resultsProbiotic treatment exhibited a trend towards reduced tumor volume and improved survival, although not statistically significant (p > 0.05). Key immune mediators showed significant changes, with TGF-β, IL-10, and IDO expressions downregulated and TNF-α and FasL upregulated in the probiotic group (P < 0.01). Histopathological analysis revealed reduced pleomorphism, mitotic activity, and focal necrosis in the probiotic group.

conclusionThe findings indicate that L. reuteri PTCC1058 may modulate the tumor microenvironment and influence immune responses in breast cancer. While further research is necessary to validate these results, probiotics could represent a complementary therapeutic strategy in breast cancer treatment.

Indexed as

Breast NeoplasmsMammary Neoplasms, ExperimentalProbioticsAnimalsCell Line, TumorDisease Models, AnimalFemaleGene Expression Regulation, NeoplasticInterleukin-10Limosilactobacillus reuteriMiceMice, Inbred BALB CTumor MicroenvironmentInterleukin-10Breast cancerImmunotherapyLactobacillus reuteriProbioticsTumor microenvironment

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.