ArticleCommunications biology2025
A second photoactivatable state of the anion-conducting channelrhodopsin GtACR1 empowers persistent activity.
Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- IR Spectroscopy: From Experimental Spectra to High-Resolution Structural Analysis by Integrating Simulations and Machine Learning.The journal of physical chemistry. B · 2025Article
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Authors and funding
8 authors.
Funding
Abstract
Optogenetics is a method to regulate cells, tissues and organisms using light. It is applied to study neurons and to develop diagnostic and therapeutic tools for neuron-related diseases. The cation-conducting channelrhodopsin ChR2 triggers photoinduced depolarization of neuronal cells but generates lower ion currents due to the syn-pathway of its branched photocycle. In contrast, the homologous anion-conducting ACR1 from Guillardia theta (GtACR1), exhibits high photocurrents. Here, we investigate the mechanistic cause for the observed high photocurrents in GtACR1 using FTIR spectroscopy. Unexpectedly, we discovered that the O intermediate of GtACR1 is photoactivable, allowing for fast and efficient channel reopening. Our vibrational spectra show a photocyclic reaction sequence after O excitation similar to the ground state photocycle but with slightly altered channel conformation and protonation states. Our results provide deeper insights into the gating mechanism of channelrhodopsins and pave the way to advance the development of optimized optogenetic tools in future.
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