Evidence map›Paper›PMID 40781588›Full record

ArticleBMC plant biology2025

Comparative physiological and transcriptomic analyses identify computationally predicted key genes and regulatory pathways in non-heading Chinese cabbage under heat stress.

Wei Liu, Zhangyang Dai, Jingyi Jia, Xiaofeng Li, Hongfang Zhu, Xianzhao Kan, Bo Zhu

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Wei Liu *Key Laboratory for the Conservation and Utilization of Important Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, 241000, Anhui Province, China.
Zhangyang Dai *Key Laboratory for the Conservation and Utilization of Important Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, 241000, Anhui Province, China.
Jingyi JiaKey Laboratory for the Conservation and Utilization of Important Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, 241000, Anhui Province, China.
Xiaofeng LiShanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.
Hongfang ZhuShanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.
Xianzhao KanKey Laboratory for the Conservation and Utilization of Important Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, 241000, Anhui Province, China. xianzhao@ahnu.edu.cn.
Bo ZhuKey Laboratory for the Conservation and Utilization of Important Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, 241000, Anhui Province, China. zhubo@ahnu.edu.cn.

Funding

key science and technology project of Shanghai Science and Technology Commission 23N11900200
6 · The paper itself

Abstract

backgroundNon-heading Chinese cabbage (Brassica campestris (syn. Brassica rapa) ssp. chinensis) (NHCC) is an important vegetable crop with economic benefits and is widely cultivated worldwide. Heat stress disrupts intracellular homeostasis, resulting in significant growth inhibition of buds and roots, severe retardation of growth and development, and even death. However, there are relatively few studies on the molecular response mechanism of NHCC to heat stress.

resultsIn this study, RNA sequencing (RNA-seq) technology was employed to investigate the transcriptome data of the heat-tolerant variety "SHI" and the heat-sensitive variety "aijiaohuang" ("AJH") under high temperature. Differentially expressed genes (DEGs), including transcription factors (TFs), genes related to hormone synthesis, and genes related to protein processing in the endoplasmic reticulum, were identified in both varieties. The combination of multi-sample RNA-seq data and weighted gene co-expression network analysis (WGCNA) identified four key modules, namely the darkgreen module, the yellow module, the blue module, and the turquoise module, and core genes within them, including MYB-related, HSP20 and DBB etc., were mined.

conclusionOur findings suggest that protein processing in the endoplasmic reticulum, photosynthesis, and plant hormone signaling are crucial for "SHI"'s response to heat stress and some candidate genes such as MYB-related, HSP20 and DBB were identified. We validated our results with qRT-PCR, reinforcing the reliability of our data. These insights deepen our understanding of the physiological and molecular bases of NHCC heat tolerance and identify key genes for further molecular investigation.

Indexed as

BrassicaHeat-Shock ResponseTranscriptomeGene Expression ProfilingGene Expression Regulation, PlantGene Regulatory NetworksGenes, PlantSequence Analysis, RNATranscription FactorsTranscription FactorsGene expressionHeat stressNon-heading chinese cabbageTranscription factorsWGCNA analyses

Identifiers

PMID40781588
PMCPMC12333117

What Socratic holds

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LicenceCC BY-NC-ND
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