ArticleJournal of inflammation research2025
Unveiling the Temporal Dynamics and Molecular Regulation Profiles of Neutrophil Extracellular Traps Following Spinal Cord Injury.
Article in Journal of inflammation research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Advancing Spinal Cord Injury Repair: The Role of Conductive Hydrogels in Neurotissue Engineering.International journal of nanomedicine · 2025Review
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Spinal cord injury (SCI) initiates secondary inflammatory processes that exacerbate tissue damage, with neutrophil extracellular traps (NETs) playing a significant role in amplifying these cascades. This study aimed to explore the temporal dynamics and key regulatory genes of NET formation in SCI to identify therapeutic targets. Methods: We integrated two transcriptomic datasets from the GEO database to identify differentially expressed NETs-related genes (NRGs) in SCI. WGCNA identified SCI-related modules, while GSVA assessed NET formation dynamics. Publicly available single-cell RNA sequencing data from the GEO database determined cell-specific expression patterns of key NRGs. Findings were validated through immunofluorescence, Western blot, and qPCR in a mouse SCI model. Regulatory networks were constructed, and potential therapeutic compounds were predicted using DSigDB and molecular docking. Results: We identified seven key NRGs (Casp1, Ccl3, Fcgr2b, Itgam, Itgb2, Tlr2, Tlr4) in SCI. GSVA revealed peak NET score at day 1 post-injury, with attenuation at days 3 and 7. Single-cell transcriptome analysis demonstrated predominant expression of these key genes in neutrophils during the acute phase, most prominently at 1 day post-injury, which coincides with the most pronounced neutrophil infiltration. Immunofluorescence and Western blot analyses confirmed significantly elevated NET formation at 1 day post-SCI. qPCR verified the expression of all key NRGs. Regulatory network analysis identified CHD1 as an important transcription factor governing NET formation, while miRNA-mRNA network construction revealed sophisticated post-transcriptional regulation mechanisms. Drug prediction analysis identified atorvastatin as a promising therapeutic candidate with strong binding affinity to multiple key NET-related proteins. Conclusion: Our study provides insights into the temporal dynamics and molecular mechanisms of NET formation after SCI, identifying potential therapeutic targets to mitigate neutrophil-mediated secondary injury and improve functional outcomes.
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Registered trials
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