Evidence mapPaperPMID 40790939Full record

ArticleCNS neuroscience & therapeutics2025

Gastrodin Attenuates Cerebral Ischemia-Reperfusion Injury by Enhancing Mitochondrial Fusion and Activating the AMPK-OPA1 Signaling Pathway.

Zihan Liu, Zeyu Han, Wenshuai Bao, Yihan Guo, Yuan Yuan, Jianming Cheng, Jie Zhang, Yang Hu

Abstract read
In one paragraph

Article in CNS neuroscience & therapeutics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zihan LiuJiangsu Province Engineering Research Center of Classical Prescription, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
Zeyu HanXian Jiaotong-Liverpool University, XJTLU Wisdom Lake Academy of Pharmacy, Suzhou, China.
Wenshuai BaoJiangsu Province Engineering Research Center of Classical Prescription, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
Yihan GuoSchool of Traditional Chinese Medicines, China Pharmaceutical University, Nanjing, China.
Yuan YuanJiangsu Province Engineering Research Center of Classical Prescription, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
Jianming ChengJiangsu Province Engineering Research Center of Classical Prescription, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
Jie ZhangXian Jiaotong-Liverpool University, XJTLU Wisdom Lake Academy of Pharmacy, Suzhou, China.
Yang HuJiangsu Province Engineering Research Center of Classical Prescription, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.ORCID 0000-0002-1759-5085

Funding

Jiangsu Province Traditional Chinese Medicine Science and Technology Development Plan Project MS2024011National Key Research and Development Program of China 2023YFC2308200the Fund of Traditional Chinese Medicine Institute of Anhui Dabie Mountain TCMADM-2024-01the National Natural Science Foundation 81803391the Nature Science Foundation of Jiangsu Higher Education Institutions of China 23KJA350003
6 · The paper itself

Abstract

backgroundCerebral ischemia-reperfusion (I/R) injury is a critical pathological process in stroke, characterized by disrupted energy metabolism, inflammatory responses, and mitochondrial dysfunction. Targeting mitochondrial dynamics presents promising strategies for alleviating brain injury. This study investigates the role and mechanism of Gastrodin (Gas) in regulating mitochondrial dynamics and mitigating cerebral I/R injury via activation of the AMPK-OPA1 signaling pathway.

methodsAn in vitro oxygen-glucose deprivation/reperfusion (OGD/R) model and an in vivo middle cerebral artery occlusion/reperfusion (MCAO/R) model were used to assess the effects of Gas on inflammation, mitochondrial function, and energy metabolism. Immunofluorescence, western blotting (WB), reverse-transcription PCR (RT-PCR), JC-1 staining, and molecular docking techniques were employed for analysis.

resultsGas activated the AMPK-OPA1 signaling pathway, promoting mitochondrial fusion, restoring membrane potential, enhancing ATP production, and rebalancing NAD

conclusionGas alleviates cerebral I/R injury by regulating mitochondrial dynamics via the AMPK-OPA1 signaling pathway. These findings provide a theoretical basis for the therapeutic application of Gas in stroke and offer new insights into mitochondrial-targeted treatment strategies.

Indexed as

AMP-Activated Protein KinasesBenzyl AlcoholsBrain IschemiaGlucosidesMitochondrial DynamicsNeuroprotective AgentsReperfusion InjuryAnimalsInfarction, Middle Cerebral ArteryMaleMiceMice, Inbred C57BLMitochondriaRatsRats, Sprague-DawleySignal TransductionAMP-Activated Protein KinasesBenzyl AlcoholsgastrodinGlucosidesNeuroprotective AgentsAMPK‐OPA1cerebral ischemia–reperfusion injuryGastrodinmitochondrial fusionoxygen–glucose deprivation/reperfusion

Identifiers

PMID40790939
PMCPMC12339906

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.