Evidence map›Paper›PMID 40794244›Full record

ArticleMolecular biology reports2025

Regulation of transglutaminase-2 and related miRNAs expression by calorie restriction in aorta of aging female mice.

Elif Öztemiz, Soner Dogan, Atakan Ayden, Aysenur Dogan, Nazim Arda Keles, Bilge Guvenc Tuna

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Elif ÖztemizDepartment of Biophysics, School of Medicine, Yeditepe University, Istanbul, Turkey.ORCID http://orcid.org/0000-0001-8002-7921
Soner DoganDepartment of Medical Biology, School of Medicine, Yeditepe University, Istanbul, Turkey.ORCID http://orcid.org/0000-0002-7762-8109
Atakan AydenDepartment of Medical Biology, School of Medicine, Yeditepe University, Istanbul, Turkey.ORCID http://orcid.org/0000-0001-6275-0403
Aysenur DoganDepartment of Medical Biology, School of Medicine, Yeditepe University, Istanbul, Turkey.ORCID http://orcid.org/0000-0002-9572-7979
Nazim Arda KelesDepartment of Medical Biology, School of Medicine, Yeditepe University, Istanbul, Turkey.ORCID http://orcid.org/0000-0001-7118-1003
Bilge Guvenc TunaDepartment of Biophysics, School of Medicine, Yeditepe University, Istanbul, Turkey. bilge.tuna@yeditepe.edu.tr.ORCID http://orcid.org/0000-0003-1348-1336

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTransglutaminase-2 (TG2) plays a key role in age-related vascular stiffness. This study aims to evaluate the effects of calorie restriction (CR) on TG2 mRNA and protein levels and identify differentially expressed microRNAs (miRNA) predicted to target TG2. METHODS AND

resultsFemale mice were assigned to ad libitum (AL), chronic CR (CCR), and intermittent CR (ICR) groups for 80 weeks. Expression levels of miRNAs predicted to target TG2 (miR-423-5p, miR-700-5p, miR-484, miR-7048-5p, and miR-7053-5p) were identified in blood and aorta samples. TG2 mRNA expression levels in the aorta were analyzed. A similar trend was observed between blood and aorta samples for miR-423-5p, miR-484, and miR-700-5p. TG2 mRNA expression was significantly lower in the ICR-R group compared to the AL group. TG2 protein levels  in aorta were lower in the CCR group than that of in the AL group in old age. Endothelial nitric oxide synthase (eNOS) protein levels were also determined owing to their role in NO-dependent TG2 regulation and were lowest in the CCR group, with an overall decline in aging mice.

conclusionsThe CR intervention reveals protective potential against vascular stiffness, as TG2 levels were lower in the aorta of aging CR mice. These findings provide translational insights into the epigenetic regulation of TG2 by CR in vascular aging.

Indexed as

AgingAortaCaloric RestrictionGTP-Binding ProteinsMicroRNAsTransglutaminasesAnimalsFemaleGene Expression RegulationMiceNitric Oxide Synthase Type IIIProtein Glutamine gamma Glutamyltransferase 2RNA, MessengerGTP-Binding ProteinsMicroRNAsNitric Oxide Synthase Type IIIProtein Glutamine gamma Glutamyltransferase 2RNA, MessengerTransglutaminasesAgingChronic calorie restrictionIntermittent calorie restrictionMicroRNATransglutaminase-2Vascular stiffness

Identifiers

PMID40794244

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.