Evidence map›Paper›PMID 40794355›Full record

ArticleOdontology2026

Calcitonin promotes osteogenic differentiation through CREB-mediated transcriptional upregulation of osteopontin in human periodontal ligament stem cells.

Shuang Qin, Meineng Huang, Xinyu Yang, Chen Li, Xuanyi Ma, Zhizen Quan, Yibo Wei

Abstract read
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In one paragraph

Article in Odontology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shuang Qin *Department of Stomatology, Changhai Hospital, Naval Medical University, 168 Changhai Rd, Shanghai, 200433, People's Republic of China.
Meineng Huang *Department of Stomatology, Changhai Hospital, Naval Medical University, 168 Changhai Rd, Shanghai, 200433, People's Republic of China.
Xinyu YangDepartment of Stomatology, Changhai Hospital, Naval Medical University, 168 Changhai Rd, Shanghai, 200433, People's Republic of China.
Chen LiDepartment of Stomatology, Changhai Hospital, Naval Medical University, 168 Changhai Rd, Shanghai, 200433, People's Republic of China.
Xuanyi MaDepartment of Stomatology, Changhai Hospital, Naval Medical University, 168 Changhai Rd, Shanghai, 200433, People's Republic of China.
Zhizen QuanDepartment of Stomatology, Changhai Hospital, Naval Medical University, 168 Changhai Rd, Shanghai, 200433, People's Republic of China. quanzz@smmu.edu.cn.
Yibo WeiDepartment of Stomatology, Changhai Hospital, Naval Medical University, 168 Changhai Rd, Shanghai, 200433, People's Republic of China. weiyibo1982@smmu.edu.cn.ORCID http://orcid.org/0000-0003-0329-6129

Funding

National Natural Science Foundation of China 81800988Natural Science Foundation of Shanghai Municipality 18ZR1438300
6 · The paper itself

Abstract

Osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) facilitates the neogenesis of alveolar bone and contribute to the alveolar bone repair. Calcitonin (CT) has been reported to promote osteogenic differentiation in osteoblast, but its effects and mechanism on human PDLSCs remain obscure. In this study, alizarin red staining and western blot were used to evaluate the osteogenic differentiation and the expression level of cAMP-response element-binding protein (CREB), phosphorylated CREB (pCREB), and osteopontin (OPN) in human PDLSCs. The transcriptional regulation of CREB on OPN expression was detected by chromatin immunoprecipitation (ChIP) and luciferase reporter assays. In human PDLSCs, CT overexpression significantly enhanced the expression of OPN and strengthened the mineralization, and upregulated the expression level of CREB and pCREB. The mineralized deposit and expression of OPN promoted by CT were reduced by inhibiting the expression or activity of CREB. CREB could directly bind to OPN promoter and transcriptionally regulate OPN expression. Inhibiting the expression of OPN reversed the cell osteogenic differentiation promoted by CREB. Our study supports that CT can promote osteogenic differentiation of human PDLSCs through enhancing the expression and phosphorylation of CREB, subsequently transcriptionally regulating OPN expressions.

Indexed as

CalcitoninCell DifferentiationCyclic AMP Response Element-Binding ProteinOsteogenesisOsteopontinPeriodontal LigamentStem CellsBlotting, WesternCells, CulturedChromatin ImmunoprecipitationHumansPhosphorylationUp-RegulationCalcitoninCyclic AMP Response Element-Binding ProteinOsteopontinCalcitonincAMP response element-binding proteinHuman periodontal ligament stem cellOsteogenic differentiationOsteopontin

Identifiers

PMID40794355

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.