Evidence map›Paper›PMID 40796239›Full record

ArticleGlycobiology2025

Evaluation of multiplexed liquid glycan Array (LiGA) for serological detection of glycan-binding antibodies.

Revathi Reddy, Eric Carpenter, Anne Halpin, Mirat Sojitra, Chuanhao Peng, Guilherme Meira Lima, Xiaochao Xue, Kejia Yan, Jean Pearcy, Maria Ellis and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Glycobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Universal Baseline forbioRxiv : the preprint server for biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Revathi ReddyDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
Eric CarpenterDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
Anne HalpinDepartment of Chemistry, University of Alberta, 11227 Saskatchewan Dr. Edmonton, AB T6G 2G2, Canada.
Mirat SojitraDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
Chuanhao PengDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
Guilherme Meira LimaDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
Xiaochao XueDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
Kejia YanDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
Jean PearcyDepartment of Pediatrics, Edmonton Clinic Health Academy, University of Alberta, 11405 87 Avenue, Edmonton, AB T6G 1C9, Canada.
Maria EllisDepartment of Pediatrics, Edmonton Clinic Health Academy, University of Alberta, 11405 87 Avenue, Edmonton, AB T6G 1C9, Canada.
Bruce MotykaDepartment of Chemistry, University of Alberta, 11227 Saskatchewan Dr. Edmonton, AB T6G 2G2, Canada.
Todd L LowaryDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
Lori WestDepartment of Chemistry, University of Alberta, 11227 Saskatchewan Dr. Edmonton, AB T6G 2G2, Canada.
Ratmir DerdaDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.ORCID 0000-0003-1365-6570

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We test the performance of the multiplexed liquid glycan array (LiGA) technology in serological assays. Specifically, we use LiGA to detect ABO blood group antibodies in human serum. This LiGA, which we name ABO-LiGA, contains ABO blood group trisaccharide glycans with an ethylazido aglycone conjugated to groups of ten multi-barcoded M13 particles carrying dibenzocyclooctyne (DBCO) on p8 proteins. ELISA clonal binding assays to anti-A/B antibodies confirmed the functional performance of ABO-clones and aligned with next-generation sequencing (NGS) of the mixed clones. Multiple DNA-barcoded technical replicates in LiGA allow for quantification of reproducibility and robustness as determined by the Z'-score using NGS. We then tested ABO-LiGA for specific detection of IgG and IgM anti-A and anti-B IgG and IgM antibodies in human serum samples. Comparison of antibody binding responses in sera from 31 healthy donors to ABO-LiGA with an ABO-Luminex-based method revealed consistent responses to LiGA-ABO but also minor deficiencies of ABO-LiGA such as low robustness of the current assay format and a limited ability to detect low intensity antibody responses. Some results point to undesired interactions of serum antibodies with small-footprint glycans conjugated to phage via the bulky DBCO moiety. This report illuminates the path for future development of LiGA-based serological assays and suggests the need to develop alternative methods for conjugating glycans to phage to avoid liabilities of the hydrophobic DBCO moiety.

Indexed as

ABO Blood-Group SystemImmunoglobulin GPolysaccharidesHumansImmunoglobulin MABO Blood-Group SystemImmunoglobulin GImmunoglobulin MPolysaccharidesanti-glycan antibodiesblood groupsliquid glycan arrayserological assay

Identifiers

PMID40796239
PMCPMC12596280

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.