Evidence map›Paper›PMID 40797206›Full record

ReviewMicrobial cell factories2025

Engineering Bacillus subtilis for high-value bioproduction: recent advances and applications.

Zi-Yan Liu, Xiao-Zheng Yu

Abstract readReview
In one paragraph

Review in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Review
  6. Review
  7. Review
  8. Article
  9. Synthetic biology for heparin biomanufacturing.Bioresources and bioprocessing · 2026
    Review
  10. Review
  11. Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Zi-Yan LiuCollege of Biological and Environmental Engineering, Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Zhejiang Shuren University, Hangzhou, 310015, China.
Xiao-Zheng YuCollege of Biological and Environmental Engineering, Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Zhejiang Shuren University, Hangzhou, 310015, China. 601824@zjsru.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacillus subtilis, a key model strain in microbial physiology and biochemistry research, is non-pathogenic, secretes proteins efficiently, and has a clear genetic background, making it an ideal host for producing heterologous proteins, such as small molecules, bulk chemicals, enzymes, and functional peptides. In recent years, the synthetic biotechnology and metabolic engineering methods for B. subtilis have advanced rapidly, providing excellent tools and theoretical references for producing target products using this chassis cell. This review systematically summarizes strain optimization strategies via screening and modification of wild-type B. subtilis strains as expression hosts, promoter engineering, fine-tuning gene expression based on proteases and molecular chaperones, plasmid-based expression systems regulation, as well as its applications. Detailed analysis was conducted from a novel perspective on the comparison between B. subtilis and E. coli as hosts for biological production. Furthermore, the review delves into important considerations and future research directions for optimizing B. subtilis expression systems in industrial production. With the development of gene editing and synthetic biology technologies, and the continuous optimization of strain improvement strategies, B. subtilis will demonstrate tremendous potentials as a major microbial cell factory for the industrial production of protein products.

Indexed as

Bacillus subtilisMetabolic EngineeringEscherichia coliGene EditingGenetic EngineeringIndustrial MicrobiologyPlasmidsPromoter Regions, GeneticSynthetic BiologyBacillus subtilisHeterologous proteinsIndustrial bioproductionPromoter engineeringSynthetic biotechnology

Identifiers

PMID40797206
PMCPMC12341298

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.