Evidence map›Paper›PMID 40799387›Full record

ArticleiScience2025

Cargo-selective regulation of clathrin-mediated endocytosis by AMP-activated protein kinase.

Laura A Orofiamma, Ralph Christian Delos Santos, Ayshin Mehrabi, Nikol Leshchyshyn, Geoffrey G Hesketh, Sadia Rahmani, Aesha Patel, Farnaz Fekri, Rehman Ata, Colin D H Ratcliffe and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Laura A OrofiammaDepartment of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada.
Ralph Christian Delos SantosDepartment of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada.
Ayshin MehrabiDepartment of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada.
Nikol LeshchyshynDepartment of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada.
Geoffrey G HeskethCentre for Systems Biology, Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON, Canada.
Sadia RahmaniDepartment of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada.
Aesha PatelDepartment of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada.
Farnaz FekriDepartment of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada.
Rehman AtaDepartment of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada.
Colin D H RatcliffeDepartment of Biochemistry, McGill University, Montreal, QC, Canada.
Mathieu J F CrupiDivision of Cancer Biology and Genetics, Sinclair Cancer Research Institute, and Department of Pathology and Molecular Medicine, Queen's University, Kingston, ON, Canada.
Lois MulliganDivision of Cancer Biology and Genetics, Sinclair Cancer Research Institute, and Department of Pathology and Molecular Medicine, Queen's University, Kingston, ON, Canada.
Morag ParkDepartment of Biochemistry, McGill University, Montreal, QC, Canada.
Anne-Claude GingrasCentre for Systems Biology, Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON, Canada.
Costin N AntonescuDepartment of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The cell surface abundance of many proteins is controlled by clathrin-mediated endocytosis (CME). CME is driven by the assembly of clathrin and other proteins on the inner leaflet of the plasma membrane into clathrin-coated pits (CCPs). Regulation of CCP dynamics allows for control of the function of specific cell surface proteins, impacting a range of cellular outcomes. AMP-activated protein kinase (AMPK) becomes activated upon metabolic insufficiency and facilitates cellular adaptation to nutrient stress. Here, we examined how AMPK regulates CME and the cell surface membrane traffic of β1-integrin. We find that AMPK controls CCP dynamics and regulates the abundance of the endocytic adaptor protein Dab2 within CCPs in a manner that requires the GTPase Arf6, thus selectively promoting the CCP recruitment and internalization of β1-integrin. This study reveals a signaling pathway for cargo-selective metabolic regulation of CME by AMPK that impacts the function of cell surface proteins such as integrins.

Indexed as

BiochemistryCell biology

Identifiers

PMID40799387
PMCPMC12341638

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.