ArticleSynthetic biology (Oxford, England)2025
A Golden Gate compatible system for continuous directed evolution in
Article in Synthetic biology (Oxford, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Directed evolution is a technique that allows for protein engineering without prior knowledge. Continuous directed evolution employs gene-specific hypermutation tied to functional selection within a single cell, enabling a broad search of sequence space for gene variants with improved or novel functions. However, currently available techniques for continuous directed evolution can be inflexible or laborious to establish. To address this issue, we present a modular toolkit for deaminase-fused viral RNA polymerase continuous directed evolution, based on Golden Gate assembly. We include an alternative RNA polymerase from phage SP6 and show that it can introduce gene-specific mutations. This work builds on the available repertoire of synthetic biology techniques, brings accessibility and versatility to directed evolution, and enables researchers to build custom and complex plasmids for their own evolutionary campaigns.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.