Evidence map›Paper›PMID 40802133›Full record

ArticleIn vitro cellular & developmental biology. Animal2025

Lactiplantibacillus plantarum promotes lactoferrin synthesis and secretion in bovine mammary epithelial cells through STAT3 and AP-1 transcription factor pathways.

Jinyu Zhou, Shuai Lian, Zijian Geng, Yuejie Yang, Rui Wu, Jianfa Wang

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jinyu ZhouCollege of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No.5 Xinfeng Road, Daqing, 163319, P. R. China.
Shuai LianCollege of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No.5 Xinfeng Road, Daqing, 163319, P. R. China.
Zijian GengCollege of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No.5 Xinfeng Road, Daqing, 163319, P. R. China.
Yuejie YangCollege of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No.5 Xinfeng Road, Daqing, 163319, P. R. China. yangyuejie1997@163.com.
Rui WuCollege of Biology and Agriculture, Jiamusi University, Jiamusi, 154007, China. fuhewu@126.com.ORCID http://orcid.org/0000-0002-9170-0517
Jianfa WangCollege of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No.5 Xinfeng Road, Daqing, 163319, P. R. China. wjflw@sina.com.

Funding

Heilongjiang Province's "Jie Bang Gua Shuai" Science and Technology Research Project 2023ZXJ02B03Natural Science Foundation of Heilongjiang Province TD2022C005the National Natural Science Foundation of China 32172814the National Natural Science Foundation of China 32172937
6 · The paper itself

Abstract

Probiotics can support the immune function of dairy cows and contribute to the synthesis of milk components in mammary gland tissue. Bovine lactoferrin (bLF) possesses immune-regulating and nutritional properties; however, the impact of probiotics on bLF remains unclear. This study aimed to investigate whether probiotics can enhance the synthesis and secretion of bLF in the mammary gland, with a particular focus on the specific mechanisms by which Lactiplantibacillus plantarum (L. plantarum) regulates bLF. Primary bovine mammary epithelial cells (BMECs) were cultured in six-well plates and treated with various types of probiotics. The expression of bLF was evaluated using quantitative real-time PCR (qRT-PCR), Western blot, and enzyme-linked immunosorbent assay (ELISA). The expression of transcription factors associated with the bLF promoter region, specifically, was analyzed through qRT-PCR and Western blot. Lacticaseibacillus rhamnosus (L. rhamnosus), Streptococcus thermophilus (S. thermophilus), Bifidobacterium (Bifido.), and L. plantarum upregulated bLF gene and protein expression to varying extents, with L. plantarum exhibiting the most pronounced effect. Furthermore, L. plantarum was found to regulate the expression of phosphorylated STAT3 and AP-1. These findings indicate that probiotics can influence the expression of bLF in mammary gland tissue. Additionally, L. plantarum modulates the production of bLF via the STAT3 and AP-1 transcription factor pathways.

Indexed as

Epithelial CellsLactiplantibacillus plantarumLactoferrinMammary Glands, AnimalSTAT3 Transcription FactorTranscription Factor AP-1AnimalsCattleFemaleGene Expression RegulationProbioticsSignal TransductionLactoferrinSTAT3 Transcription FactorTranscription Factor AP-1Bovine lactoferrinBovine mammary epithelial cellsGene expression regulationLactiplantibacillus plantarumProbiotics

Identifiers

PMID40802133

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.