Evidence map›Paper›PMID 40805067›Full record

ArticleAnimals : an open access journal from MDPI2025

Single-Nucleus RNA Sequencing and Spatial Transcriptomics Reveal Cellular Heterogeneity and Intercellular Communication Networks in the Hypothalamus-Pituitary-Ovarian Axis of Pregnant Mongolian Cattle.

Yanchun Bao, Fengying Ma, Chenxi Huo, Hongxia Jia, Yunhan Li, Xiaoyi Yang, Jiajing Liu, Pengbo Gu, Caixia Shi, Mingjuan Gu and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yanchun BaoCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Fengying MaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Chenxi HuoCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.ORCID 0009-0004-8988-4638
Hongxia JiaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.ORCID 0009-0009-3913-2944
Yunhan LiCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Xiaoyi YangCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Jiajing LiuCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Pengbo GuCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Caixia ShiCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Mingjuan GuCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.ORCID 0000-0002-8244-9519
Lin ZhuCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Yu WangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.
Bin LiuInner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Inner Mongolia Agricultural University, Hohhot 010018, China.
Risu NaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Wenguang ZhangCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.

Funding

Double First-Class Construction Funds for Inner Mongolia Agricultural University No. BZX202201, CG202410, CG202423, NDYB2022-1Fundamental Research Founds for Inner Mongolia Autonomous Region Direct Affiliated Universities No. BR251026Inner Mongolia Open Competition Project No. 2022JBGS0025
6 · The paper itself

Abstract

The hypothalamus-pituitary-ovarian (HPO) axis orchestrates reproductive functions through intricate neuroendocrine crosstalk. Here, we integrated single-nucleus RNA sequencing (snRNA-seq) and spatial transcriptomics (ST) to decode the cellular heterogeneity and intercellular communication networks in the reproductive systems of pregnant Mongolian cattle. We retained a total of 6161 high-quality nuclei from the hypothalamus, 14,715 nuclei from the pituitary, and 26,072 nuclei from the ovary, providing a comprehensive cellular atlas across the HPO axis. In the hypothalamus, neurons exhibited synaptic and neuroendocrine specialization, with glutamatergic subtype Glut4 serving as a TGFβ signaling hub to regulate pituitary feedback, while GABAergic GABA1 dominated PRL signaling, likely adapting maternal behavior. Pituitary stem cells dynamically replenished endocrine populations via TGFβ, and lactotrophs formed a

Indexed as

cattlehypothalamic–pituitary–ovarian axisreproductive regulationsingle-nucleus RNA sequencingspatial transcriptomics

Identifiers

PMID40805067
PMCPMC12345466

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.