Evidence map›Paper›PMID 40815365›Full record

ArticleProbiotics and antimicrobial proteins2026

Recombinant Bacillus subtilis Displaying DHAV-1 VP1 Protein as a Dual-Function Probiotic: Evaluation of Immunological Efficacy, Growth Performance, Antioxidant Capacity, and Intestinal Health in Cherry Valley Ducks.

Bin Chen, Yicen Tang, Lihui Huang, Zhenhua Wang, Jianzhen Li, Mengwei Zhang, Yang Yang, Dongmei Zhang, Bo Jing, Yan Zeng and 3 more

Abstract read
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Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Bin Chen *College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Yicen Tang *College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Lihui Huang *College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Zhenhua Wang *College of Animal Husbandry and Veterinary, Chengdu Agricultural College, Chengdu, 611130, China.
Jianzhen LiCollege of Animal Husbandry and Veterinary, Chengdu Agricultural College, Chengdu, 611130, China.
Mengwei ZhangCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Yang YangCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Dongmei ZhangCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Bo JingCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Yan ZengCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Xueqin NiCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Jinlong YangChongqing Academy of Animal Sciences, Chongqing, 402460, China.
Kangcheng PanCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. pankangcheng@sicau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Duck hepatitis A virus type 1 (DHAV-1) demonstrates extremely high lethality in 3-week-old ducklings, while causing subclinical infections in adult ducks with latent pathogenicity. Our previous research developed a vaccine-functional probiotic preparation targeting DHAV-1-recombinant Bacillus subtilis RV. In the present study, we conducted a 28-day oral immunization trial in Cherry Valley ducks to evaluate the immunoprotective effects and probiotic functions of this engineered strain. Results showed that B. subtilis RV induced humoral and mucosal immune responses in ducklings, producing high levels of specific IgG and intestinal secretory IgA, demonstrating immune-enhancing capabilities. During DHAV-1 challenge tests, it significantly reduced viral loads in various organ tissues. Additionally, B. subtilis RV improved the growth performance and antioxidant capacity of ducklings. Furthermore, this engineered probiotic enhanced intestinal health by improving intestinal structure, increasing tight junction protein expression, and modulating gut microbiota composition. This study evaluates a novel vaccine-functional probiotic preparation, providing a new strategy for DHAV-1 prevention and control.

Indexed as

Bacillus subtilisCapsid ProteinsDucksHepatitis Virus, DuckPoultry DiseasesProbioticsAnimalsAntioxidantsGastrointestinal MicrobiomeIntestinesAntioxidantsCapsid ProteinsAntiviral infectionBacillus subtilisCherry Valley duckDHAV-1Microecological preparationProbiotic properties

Identifiers

PMID40815365

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.