ArticleCommunications biology2025
Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis.
Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Genomic characterization and phage cocktail suppression of a multidrug-resistantApplied and environmental microbiology · 2026Article
- Beyond Resistance Genes:Life (Basel, Switzerland) · 2026Review
- Phenotypic heterogeneity optimizes trade-offs during adaptive deployment of the type VI secretion system.PLoS biology · 2026Article
- An integrated computational-experimental approach identifies Malate Synthase G as therapeutic target to disrupt carbon metabolism in chronic Pseudomonas aeruginosa infection.Functional & integrative genomics · 2026Article
- T6SS mutants exploit itaconate to support infection of phagocytes.bioRxiv : the preprint server for biology · 2026Article
- The Putative RNA Methyltransferase Modulates T3SS Expression and Host NF-κB Activation via T6SS-Mediated Translocation inInternational journal of molecular sciences · 2026Article
- Control of flagellar gene expression by a chemotaxis receptor-like regulator in pathogenic Escherichia coli.The EMBO journal · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Pseudomonas aeruginosa, a versatile Gram-negative opportunistic pathogen, relies on multiple virulence mechanisms, including a Type III Secretion System (T3SS) and several Type VI Secretion Systems (T6SS), to establish infections. The bacterial universal second messenger cyclic di-guanylate (c-di-GMP) orchestrates the lifestyle transitions of Pseudomonas aeruginosa between motile and biofilm-associated states and influences the expression of virulence traits. While it is clear that these systems are interconnected, their precise interaction on the single-cell level has remained unclear. In this study, we use single-cell analysis to dissect the role of c-di-GMP in the heterogeneity of virulence factors in P. aeruginosa populations. Our results confirm earlier findings that on the population level, high c-di-GMP levels lead to increased formation and activity of the H1-T6SS, while negatively influencing formation and activity of the T3SS. On the single-cell level, we further characterize the virulence crosstalk within P. aeruginosa populations by presenting a cooperative relationship among T3SS and flagellum and antagonistic relationships between presence of the H1-T6SS and the T3SS as well as the flagellum. Overall, this c-di-GMP-orchestrated heterogeneity and crosstalk of virulence systems suggest a strategy to optimize survival and pathogenicity under varying environmental conditions in the framework of the motile-sessile lifestyle transition.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.