Evidence map›Paper›PMID 40820159›Full record

ArticleScientific reports2025

Network pharmacology and metabolomics reveal mathurameha, a Thai traditional Anti-Diabetic formula, enhances glucose metabolism through PI3K-AKT/AMPK/GLUT4 pathway modulation.

Subhadip Banerjee, Wuttichai Jaidee, Narawadee Rujanapun, Thidarat Duangyod, Tharakorn Maneerat, Pravaree Phuneerub, Kulwadee Malee, Siam Popluechai, Virayu Suthiphasilp, Panupong Puttarak and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Subhadip BanerjeeMedicinal Plant Innovation Center of Mae Fah Luang University, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Wuttichai JaideeMedicinal Plant Innovation Center of Mae Fah Luang University, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Narawadee RujanapunMedicinal Plant Innovation Center of Mae Fah Luang University, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Thidarat DuangyodMedicinal Plant Innovation Center of Mae Fah Luang University, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Tharakorn ManeeratMedicinal Plant Innovation Center of Mae Fah Luang University, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Pravaree PhuneerubMedicinal Plant Innovation Center of Mae Fah Luang University, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Kulwadee MaleeMedicinal Plant Innovation Center of Mae Fah Luang University, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Siam PopluechaiSchool of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Virayu SuthiphasilpDepartment of Industrial Technology and Innovation Management, Faculty of Science and Technology, Pathumwan Institute of Technology, Bangkok, 10330, Thailand.
Panupong PuttarakPhytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, 90110, Songkhla, Thailand.
Poonsit HiransaiCenter of Excellence in Marijuana, Hemp, and Kratom, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
Surat LaphookhieoCenter of Chemical Innovation for Sustainability (CIS) and School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Salfarina RamliFaculty of Pharmacy, Universiti Teknologi MARA Cawangan Selangor, Puncak Alam, 42300, Selangor, Malaysia.
Wim Vanden BergheLaboratory for Protein Chemistry, Proteomics & Epigenetic Signalling (PPES), Department of Biomedical Sciences, University of Antwerp, Wilrijk, 2610, Belgium.
Geoffrey A CordellNatural Products Inc., Evanston, IL, 60201, USA.
Rawiwan CharoensupMedicinal Plant Innovation Center of Mae Fah Luang University, Mae Fah Luang University, Chiang Rai, 57100, Thailand. rawiwan.cha@mfu.ac.th.

Funding

Mae Fah Luang University POSTDOCTORAL FELLOWSHIP , GRANT NO. 04/2025Office of the Permanent Secretary, Ministry of Higher Education, Science, Research and Innovation, Thailand F01- 683R-17-046Thailand Science Research and Innovation Fund DBG6280007The Hub of Knowledge: Herbs for Sustainable Health and Well-being Consortium and supported by the National Research Council of Thailand N35E680089
6 · The paper itself

Abstract

Traditional herbal formulations offer promising avenues for diabetes management by targeting multiple molecular pathways. Mathurameha (MT), a polyherbal preparation, has been historically used for its antidiabetic potential. However, its molecular mechanisms remain largely unexplored. FrE exhibited potent α-glucosidase inhibition (IC₅₀ 0.3 µg/mL) and significantly enhanced glucose uptake in L6 myotubes (3.67 ± 0.23-fold) and 3T3-L1 adipocytes (IC₅₀ 6.78 µg/mL). It also stimulated insulin secretion (1.42-fold), comparable to metformin (1.46-fold), and protected INS-1 pancreatic β-cells from H₂O₂-induced apoptosis (30.65 ± 3.54%) through partial caspase-3 inhibition. LC-MS-QTOF analysis identified 73 metabolites, including ellagic acid, kushenol A, gallic acid, arctiin, neoandrographolide, astilbin, paenol, muricatacin, coumarrayin, and zingerone. Network pharmacology and pathway enrichment analyses revealed key targets (GSK3β, GLUT4, PPARG, INSR, AKT2, CASP3, and MMP9) and highlighted the involvement of PI3K-AKT, AMPK, and GLUT4 signaling pathways. Gene expression analysis confirmed the upregulation of GLUT4, AMPK, IRS, PI3K, and AKT genes in L6 myotubes treated with FrE. These findings suggest that MT exerts antidiabetic effects via the PI3K-AKT/AMPK/GLUT4 signaling axis, promoting glucose uptake, insulin secretion, and β-cell protection. Future studies will focus on in vivo validation, standardization of bioactive fractions, and omics-based approaches to establish a well-defined, effective formulation for diabetes management.

Indexed as

GlucoseGlucose Transporter Type 4Hypoglycemic AgentsPlant ExtractsSignal Transduction3T3-L1 CellsAdipocytesAMP-Activated Protein KinasesAnimalsApoptosisCell LineInsulinInsulin-Secreting CellsMetabolomicsMiceMuscle Fibers, SkeletalAMP-Activated Protein KinasesGlucoseGlucose Transporter Type 4Hypoglycemic AgentsInsulinPhosphatidylinositol 3-KinasesPlant ExtractsProto-Oncogene Proteins c-aktAntidiabetic activityGlucose transportInsulin secretion enrichmentLC-MS/MS-QTOF analysisMathuramehaNetwork pharmacologyThai traditional medicineTwenty-six medicinal plant mixture

Identifiers

PMID40820159
PMCPMC12358555

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.