Evidence map›Paper›PMID 40820206›Full record

ArticleBMC microbiology2025

Heterologous expression of the M. abscessus CBASS system confers phage TM4 resistance in M. smegmatis.

Chun-Liang Wang, Ao-Fei Duan, Da-Min Pan, Yi-Ou Zhao, Jian Yang, Yi-Cheng Sun

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Chun-Liang Wang *NHC Key Laboratory of Systems Biology of Pathogens, State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 102629, China.
Ao-Fei Duan *NHC Key Laboratory of Systems Biology of Pathogens, State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 102629, China.
Da-Min Pan *NHC Key Laboratory of Systems Biology of Pathogens, State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 102629, China.
Yi-Ou ZhaoSchool of Life Science and Technology, Changchun University of Science and Technology, Changchun, Jilin, 130031, China.
Jian YangNHC Key Laboratory of Systems Biology of Pathogens, State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 102629, China. yangj@ipbcams.ac.cn.
Yi-Cheng SunNHC Key Laboratory of Systems Biology of Pathogens, State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 102629, China. sunyc@ipbcams.ac.cn.

Funding

National Key Research and Development Program of China 2023YFE0113400the CAMS Innovation Fund for Medical Sciences CIFMS2021-I2M-1-043
6 · The paper itself

Abstract

Phage therapy for multidrug-resistant Mycobacterium abscessus is constrained by the narrow lytic spectrum of phages due to unknown antiphage mechanisms. Bacteria deploy various defense strategies to prevent phage infection, but few have been comprehensively characterized in mycobacteria. Mycobacteriophage TM4 successfully delivers DNA into M. abscessus but fails to establish infection. Bioinformatic analyses predicted three candidate phage defense systems and multiple individual putative defense proteins in M. abscessus. Among them, Mab_2091, Mab_2092, and Mab_2093, designated CmaABC, are components of a cyclic oligonucleotide-based antiphage signaling system (CBASS) preventing TM4 infection. However, the inability of TM4 to form plaques even in CBASS-deficient M. abscessus implies the involvement of additional resistance mechanisms. Our findings underscore the challenges faced by mycobacteriophages in infecting M. abscessus, and highlight the complex interactions between this pathogen and its viral adversaries.

Indexed as

Bacterial ProteinsMycobacteriophagesMycobacterium abscessusMycobacterium smegmatisSignal TransductionBacterial ProteinsCBASSMycobacteriophage TM4Mycobacterium abscessusPhage resistance

Identifiers

PMID40820206
PMCPMC12359967

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.