Evidence map›Paper›PMID 40820941›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Clathrin Light Chain B Drives Hepatocellular Carcinoma Progression Through Dual Mechanisms: Small Extracellular Vesicle-Mediated Angiogenesis and the NF-κB-PCLAF Signaling Axis.

Xiaoke Sun, Junchen Guo, Ning Zhao, Guanghua Cui, Yun Bai, Meijuan Ding, Yi Xu, Yu Yang

Abstract read
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiaoke SunDepartment of Internal Medical Oncology, Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu ROAD, Harbin, 150086, P. R. China.
Junchen GuoDepartment of Cardiology, Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu ROAD, Harbin, 150086, P. R. China.
Ning ZhaoDepartment of Internal Medical Oncology, Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu ROAD, Harbin, 150086, P. R. China.
Guanghua CuiDepartment of Internal Medical Oncology, Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu ROAD, Harbin, 150086, P. R. China.
Yun BaiDepartment of Internal Medical Oncology, Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu ROAD, Harbin, 150086, P. R. China.
Meijuan DingDepartment of Internal Medical Oncology, Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu ROAD, Harbin, 150086, P. R. China.
Yi XuDepartment of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu ROAD, Harbin, 150086, P. R. China.ORCID https://orcid.org/0000-0003-2720-0005
Yu YangDepartment of Internal Medical Oncology, Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu ROAD, Harbin, 150086, P. R. China.ORCID https://orcid.org/0000-0002-8059-7016

Funding

Anhui Engineering Technology Research Center of Biochemical Pharmaceutical, Bengbu Medical University 2024SYKFD01Beijing Science and Technology Innovation Medical Development Foundation KC2023-JX-0186-FM046Beijing Xisike Clinical Oncology Research Foundation Y-QL202201-0020Education Department of Guangxi Zhuang Autonomous Region GXSWFZ202401Key Laboratory of Human Development and Disease Research, Guangxi Medical University, Education Department of Guangxi Zhuang Autonomous Region RTFY202301Key R&D Program of Heilongjiang Province GZ2024023National Natural Science Foundation of China 82472743Open Funds of State Key Laboratory of Oncology in South China HN2025-02Opening Project of Fujian Provincial Key Laboratory of Innovative Drug Target Research FJ-YW-2024KF02Opening Project of Fujian Provincial Key Laboratory of Translational Cancer Medicine TCM2024-3Opening Project of Fujian Provincial Key Laboratory of Tumor Biotherapy FJZL2023001Opening Project of Key Laboratory of Functional and Clinical Translational Medicine, Fujian Province University XMMC-OP2024012Opening Project of the Scientific and Technological Innovation Major Base of Guangxi 2022-36-Z05-GXSWBX202201Thematic Research Support Scheme of State Key Laboratory of Liver Research, The University of Hong Kong SKLLR/TRSS/2022/08
6 · The paper itself

Abstract

Clathrin light chain B (CLTB) is one of the three light chain subunits of the clathrin complex. This study aims to elucidate the role of CLTB in the pathogenesis of hepatocellular carcinoma (HCC) and its clinical implications. Clinical and bioinformatic analyses reveal marked CLTB overexpression in HCC tissues. Genetic silencing of CLTB suppresses HCC cell proliferation, migration, and invasion, whereas its overexpression exacerbates malignant phenotypes. Mechanistically, CLTB activates NF-κB signaling to upregulate PCNA clamp-associated factor (PCLAF), thereby promoting small extracellular vesicle (sEV) uptake. Given that clathrin-mediated endocytosis is the key mechanism for sEV uptake, this study further investigated the functional implications of CLTB-enriched sEVs in tumor vascular remodeling. sEV-CLTB promotes endothelial angiogenesis, disrupts vascular integrity, and induces pulmonary vascular leakage by binding SH3 domain-containing kinase-binding protein 1 (SH3KBP1) and then inhibiting SH3KBP1 ubiquitination degradation. In patient-derived xenograft (PDX) models, combined therapy of clathrin inhibitor (chlorpromazine) or SH3KBP1 silencing with sorafenib suppresses tumor growth and reduces microvascular density. This study demonstrates that CLTB promotes HCC progression through the NF-κB-PCLAF signaling axis and sEV-mediated vascular remodeling, providing a mechanistic foundation for developing combination therapies targeting CLTB.

Indexed as

Carcinoma, HepatocellularExtracellular VesiclesLiver NeoplasmsNeovascularization, PathologicNF-kappa BAngiogenesisAnimalsCell Line, TumorCell ProliferationDisease ProgressionHumansMaleMiceSignal TransductionNF-kappa Bclathrin light chain Bhepatocellular carcinomaintercellular communicationsmall extracellular vesiclesvascular permeability

Identifiers

PMID40820941
PMCPMC12622436

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.