Evidence map›Paper›PMID 40823808›Full record

ArticleNucleic acids research2025

A time-resolved framework for the recruitment of mRNP processing and assembly factors to a site of transcription.

Theresa Wechsler, Ryuta Asada, Andrew N M Dominguez, Rachel Montpetit, Julia K McCormick, Kalyn Concepcion, Ben Montpetit

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Theresa WechslerDepartment of Viticulture and Enology, University of California, Davis, CA 95616, United States.
Ryuta AsadaDepartment of Viticulture and Enology, University of California, Davis, CA 95616, United States.
Andrew N M DominguezDepartment of Viticulture and Enology, University of California, Davis, CA 95616, United States.
Rachel MontpetitDepartment of Viticulture and Enology, University of California, Davis, CA 95616, United States.
Julia K McCormickDepartment of Viticulture and Enology, University of California, Davis, CA 95616, United States.
Kalyn ConcepcionDepartment of Viticulture and Enology, University of California, Davis, CA 95616, United States.
Ben MontpetitDepartment of Viticulture and Enology, University of California, Davis, CA 95616, United States.ORCID 0000-0002-8317-983X

Funding

UC Davis MCB T32 Administrative Supplement to Recognize Excellence in Diversity, Equity, Inclusion, and Accessibility (DEIA) MentorshipT32GM007377 · NIGMS · UNIVERSITY OF CALIFORNIA DAVIS · PI CHEDIN, FREDERIC LOUIS · 1985 to 2023
$9.9M
Acquisition of Covaris E220 and Sciclone G3 systems for high throughput sequencinS10OD010786 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI COMAI, LUCA · 2012 to 2012
$311k
Modeling THO complex neurodevelopmental disorder mutations in gene expression regulation using budding yeastF31NS131037 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI WECHSLER, THERESA FAITH · 2023 to 2024
$82k
DNA Technologies and Expression Analysis CoresJapan Society for the Promotion of ScienceNational Institutes of Health Shared Instrumentation 1S10OD010786-01National Science Foundation 2140761NIGMS NIH HHS GM007377NIGMS NIH HHS T32 GM007377NIH HHS S10 OD010786NINDS NIH HHS F31 NS131037NINDS NIH HHS F31NS131037Training Program in Molecular and Cellular Biology
6 · The paper itself

Abstract

Processing and packaging of messenger ribonucleoprotein (mRNP) particles involve complex, coordinated interactions between nascent transcripts, RNA-binding proteins (RBPs), and associated factors. Despite the critical role of co-transcriptional mRNP assembly in gene expression, the temporal dynamics of this process are not well understood. Here, a live cell imaging assay is reported in Saccharomyces cerevisiae to detect recruitment of endogenous fluorescently tagged proteins to a transcriptionally active locus. Protein recruitment to an inducible integrated gene array composed of 25 transcriptional units is detected by colocalization with lacO repeats. Using arrays with two different promoters and the same coding sequence (GFA1), arrival times for a variety of mRNP processing and assembly factors were quantified. These analyses revealed Yra1, Cbp80, and Yhs7 as pioneering mRNP assembly factors. Notably, Yra1 recruitment occurs independently of the THO complex, with early localization supported by Cbp80 and the RNA recognition motif of Yra1. Altogether, this work establishes the first comprehensive temporal framework for understanding protein recruitment during co-transcriptional mRNP assembly, providing mechanistic insights into the dependencies of Yra1 recruitment.

Indexed as

RibonucleoproteinsRNA-Binding ProteinsSaccharomyces cerevisiae ProteinsTranscription, GeneticNuclear ProteinsPromoter Regions, GeneticSaccharomyces cerevisiaemessenger ribonucleoproteinNuclear ProteinsRibonucleoproteinsRNA-Binding ProteinsSaccharomyces cerevisiae ProteinsYRA1 protein, S cerevisiae

Identifiers

PMID40823808
PMCPMC12359037

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.