Evidence map›Paper›PMID 40826040›Full record

ArticleBMC plant biology2025

CHH demethylation in the ZmGST2 promoter enhances maize drought tolerance by regulating ROS scavenging and root growth.

Xiaocui Yan, Mengjie Zhang, Yuan Zhong, Tinashe Zenda, Songtao Liu, Anyi Dong, Mengyu Kou, Jialong Liu, Nan Wang, Huijun Duan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Uncovering the Role of thePlants (Basel, Switzerland) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xiaocui Yan *State Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding, Hebei, 071001, China.ORCID http://orcid.org/0000-0003-0507-1388
Mengjie Zhang *State Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding, Hebei, 071001, China.
Yuan ZhongState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding, Hebei, 071001, China.
Tinashe ZendaCrop Science Department, Faculty of Plant and Animal Sciences and Technology, Marondera University of Agricultural Sciences and Technology, P. O. Box, 35, Marondera, Zimbabwe.
Songtao LiuCollege of Agriculture and Forestry, Hebei North University, Zhangjiakou, 075000, China.
Anyi DongState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding, Hebei, 071001, China.
Mengyu KouState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding, Hebei, 071001, China.
Jialong LiuState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding, Hebei, 071001, China.
Nan WangState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding, Hebei, 071001, China. wangnan@hebau.edu.cn.ORCID http://orcid.org/0009-0006-5018-1462
Huijun DuanState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding, Hebei, 071001, China. hjduan@hebau.edu.cn.ORCID http://orcid.org/0000-0003-0900-3884

Funding

National Key Research and Development Program of china 2023YFD2301501S&T Program of Hebei 24466301Dthe National Natural Science Foundation of China 32071936
6 · The paper itself

Abstract

As an important food crop worldwide, maize is frequently impacted by drought stress. It is of great significance to study its drought resistance genes and mechanisms, in which DNA methylation modification in the promoter region plays a key role in regulating gene expression and stress resistance. In this study, using PEG6000 and 5-azadC to treat different drought-tolerant maize inbred lines (drought-tolerant R99 and drought-sensitive Mo17), combined with transcriptomic analysis, identified the gene ZmGST2 as being related to drought resistance. Subsequently, the methylation changes in its promoter region were further analyzed. The results indicated that the expression level of ZmGST2 was closely related to the methylation status of its promoter region under drought stress. Specifically, in drought-resistant R99, a decrease in methylation (demethylation) occurred within the CpG island of the ZmGST2 promoter region after drought treatment, which facilitated the expression of this gene and thereby enhanced drought resistance in maize. However, in the drought-sensitive Mo17, this demethylation change did not occur, resulting in reduced drought resistance. Further phenotypic analysis revealed that overexpression of ZmGST2 could enhance the reactive oxygen species scavenging ability in maize roots, improve root growth, and significantly enhance drought resistance. Overall, this study provides both a new candidate gene and a novel approach for molecular breeding aimed at enhancing maize drought resistance.

Indexed as

DNA MethylationDroughtsPlant ProteinsPlant RootsReactive Oxygen SpeciesZea maysDemethylationDrought ResistanceGene Expression Regulation, PlantPromoter Regions, GeneticStress, PhysiologicalPlant ProteinsReactive Oxygen SpeciesDNA methylationDrought resistanceEpigenetic regulationGlutathione metabolismReactive oxygen species

Identifiers

PMID40826040
PMCPMC12359901

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.