Evidence map›Paper›PMID 40826427›Full record

ArticleVirology journal2025

Establishment and comparison of RF-RAA and qPCR for rapid detection of Chinese soft-shelled turtle adenovirus (CSTAdV).

Feiyan Tian, Zhigao Zhan, Jianming Pei, Haili Huang, Zemao Gu

Abstract readComparative Study
In one paragraph

Article in Virology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Feiyan TianDepartment of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
Zhigao ZhanInstitute of Microbiology, Jiangxi Academy of Sciences, Nanchang, 330029, China.
Jianming PeiJiangxi Provincial Center for Agricultural Technical Extension, Nanchang, 330046, China.
Haili HuangJiangxi Provincial Center for Agricultural Technical Extension, Nanchang, 330046, China.
Zemao GuDepartment of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China. guzemao@mail.hzau.edu.cn.

Funding

Jiangxi Agriculture Research System JXARS-2025-14Key R&D Program Project of Science and Technology Bureau of Fuzhou City 2022DB25Key research and development project of Jiangxi academy of sciences 2023YSBG22006National Natural Science Foundation of China 32360701the guiding research project on agriculture, animal husbandry, and fishery in Jiangxi Province 2024-44
6 · The paper itself

Abstract

backgroundChinese soft-shelled turtle adenovirus (CSTAdV) is a new virus discovered recently that infects farmed Chinese soft-shelled turtle. In order to investigate its epizootiology and meet the requirements of timely prevention and control, it is imperative to establish an efficient diagnostic assay for CSTAdV.

resultsIn this study, on-site diagnostic real-time fluorescence Recombinase-aided amplification (RF-RAA) and real-time quantitative polymerase chain reaction (qPCR) detection methods were established based on the specific sequence of the viral DNA polymerase gene. The results showed that the sensitivity of the CSTAdV RF-RAA assay and qPCR assay was 1.0 × 10

conclusionsTherefore, the RF-RAA and qPCR assays provide two novel alternatives for simple, sensitive and specific identification of CSTAdV for pathogen screening in the field and quantitative analysis in the laboratory.

Indexed as

AdenoviridaeAdenoviridae InfectionsMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesReal-Time Polymerase Chain ReactionTurtlesAnimalsChinaDNA, ViralRecombinasesSensitivity and SpecificityDNA, ViralRecombinasesAided amplificationChinese softRapid detectionRealRecombinaseShelled turtle adenovirusTime quantitative polymerase chain reaction

Identifiers

PMID40826427
PMCPMC12362846

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.