Evidence map›Paper›PMID 40830755›Full record

ArticleBMC genomics2025

Genome-wide identification and characterization of the ubiquitin-specific protease (USP) gene family in cattle: primary analysis of muscle-specific USP genes and their influence on myogenesis.

Yufei Zan, Junliang Li, Fengcheng Song, Yixuan Tang, Yuxuan Xin, Zhepei Zhang, Linjuan Wang, Lupei Zhang, Liang Du, Zhengrong Yuan and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yufei Zan *College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Junliang Li *Institute of Animal Science(IAS), Chinese Academy of Agricultural Sciences(CAAS), Beijing, 100193, China.
Fengcheng SongCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Yixuan TangCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Yuxuan XinCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Zhepei ZhangCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Linjuan WangCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Lupei ZhangInstitute of Animal Science(IAS), Chinese Academy of Agricultural Sciences(CAAS), Beijing, 100193, China.
Liang DuCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China. duliang@bjfu.edu.cn.
Zhengrong YuanCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China. zryuan@bjfu.edu.cn.
Xue GaoInstitute of Animal Science(IAS), Chinese Academy of Agricultural Sciences(CAAS), Beijing, 100193, China. gaoxue@caas.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe ubiquitin-proteasome system(UPS) is a critical biological pathway that regulates protein function and plays a pivotal role in muscle formation. Nevertheless, the current comprehension of the ubiquitin-specific protease (USP) family, an important component of the UPS, in relation to bovine myoblast development remains relatively limited. This study aims to characterize the bovine USPs and conduct a preliminary analysis of their function, to provide valuable insights for enhancing beef yield and quality.

resultsA comprehensive genome-wide analysis was conducted to explore the genetic characteristics of the USP family, which is categorized into 13 unique categories. The genetic homology of the USP family between cattle and other related species was discovered through collinearity analysis. Notably, cattle muscle tissue exhibits high expression levels of USP2, USP13, USP19, USP25, USP28, USP38, USP47, and USP53. Furthermore, the expression patterns of these genes during myogenic cell differentiation can be categorized into 4 distinct types. By designing small interfering RNA(siRNA) to interfere with the expression of USP2, USP28, USP38, and USP47 in the myogenic cell, the silencing of these genes reduced the proliferation capacity of the myogenic cell. Meanwhile, the silencing of USP2, USP28, and USP38 facilitated myogenic cell differentiation, while USP47 exerted an opposing effect.

conclusionsIn this study, a total of 53 members of USPs were successfully identified in Bos taurus. SiRNA interference experiments revealed that USP2, USP28, USP38, and USP47 are implicated in the regulation of myogenic cell proliferation and differentiation. These findings lay the foundation for further investigation into the precise roles of USPs in governing myoblast behavior and hold promise for enhancing beef quality.

Indexed as

Muscle DevelopmentUbiquitin-Specific ProteasesAnimalsCattleCell DifferentiationCell ProliferationMultigene FamilyUbiquitin-Specific ProteasesCattleDeubiquitinationMuscle developmentMyoblast proliferation and differentiationUSP

Identifiers

PMID40830755
PMCPMC12362952

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.