Evidence mapPaperPMID 40832995Full record

ArticleEuropean journal of histochemistry : EJH2025

Aloe-emodin ameliorates chronic kidney disease fibrosis by inhibiting PI3K-mediated signaling pathway.

Ming Chen, Wenhui Zhu, Yao Chen, Jingying Shang, Wenfan Wang, Xiaoming Yan, Peng Liu, Yabin Zhou

Abstract read
In one paragraph

Article in European journal of histochemistry : EJH, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ming ChenHeilongjiang University of Chinese Medicine, Harbin; Renal Division, Department of Medicine, Heilongjiang Academy of Chinese Medicine Sciences, Harbin.
Wenhui ZhuCollege of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun.
Yao ChenDepartment of Nephrology, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing.
Jingying ShangHeilongjiang University of Chinese Medicine, Harbin.
Wenfan WangCollege of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun.
Xiaoming YanRenal Division, Department of Medicine, Heilongjiang Academy of Chinese Medicine Sciences, Harbin.
Peng LiuDepartment of Nephrology, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing.
Yabin ZhouThe First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chronic kidney disease (CKD) impacts a vast number of individuals worldwide, culminating in renal fibrosis. Renal fibrosis serves as the main reason for end-stage renal failure. However, the current targeted treatment methods for renal fibrosis remain scarce. Aloe-emodin (AE) is a naturally occurring compound discovered in rhubarb and aloe. In this research, we investigated the underlying mechanisms of AE in adenine-induced mouse renal fibrosis models and TGFβ-1 stimulated renal tubular epithelial cells (HK-2). It was discovered that AE not only decelerated the decline of renal function in adenine-treated mice but also suppressed the expression of Collagen I and Fibronectin. Furthermore, network pharmacology analysis suggested that AE's treatment of renal fibrosis might function via the PI3K/Akt/GSK3β signaling pathway. In vivo and in vitro Western blot and immunofluorescence findings demonstrate that AE significantly resists the advancement of renal fibrosis by inhibiting α-smooth muscle actin (α-SMA) and vimentin. Simultaneously, findings from 740Y-P (a PI3K agonist) and siRNA (PI3K) indicate that AE inhibits the expression of the PI3K/Akt/GSK3β cascade by lowering PI3K's phosphorylation level. From a mechanistic perspective, through molecular docking and plasmid transfection, the specific base sequence of PI3K in HK-2 cells was altered for experimental validation. The outcomes illustrate that AE can directly bind with PI3K, inhibiting its activation, impeding the PI3K/Akt/GSK3β signal transmission, thereby ultimately suppressing renal fibrosis progression. In conclusion, PI3K/Akt/GSK3β is a potential therapeutic target for CKD-related renal fibrosis, making AE a promising new treatment alternative for this condition.

Indexed as

AnthraquinonesPhosphatidylinositol 3-KinasesRenal Insufficiency, ChronicSignal TransductionAnimalsCell LineFibrosisGlycogen Synthase Kinase 3 betaHumansKidneyMaleMiceMice, Inbred C57BLProto-Oncogene Proteins c-aktaloe emodinAnthraquinonesGlycogen Synthase Kinase 3 betaPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-akt

Identifiers

PMID40832995
PMCPMC12406115

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.