Evidence map›Paper›PMID 40833571›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Quantification and Comparison of Protein Distribution on the Nuclear Membrane.

Paulina Nastały, Paolo Maiuri

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Paulina NastałyDivision of Translational Oncology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland. paulina.nastaly@gumed.edu.pl.
Paolo MaiuriDepartment of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The nuclear membrane also referred as nuclear envelope (NE) encloses the nucleoplasm of the cell and acts as a barrier between the nuclear DNA and the cytoplasm. Recent studies suggest that the composition of proteins at the NE is not even throughout the whole membrane and can show zones with accumulation of some proteins inner and outer nuclear membrane protein, emerin, lamin A/C, or nesprin-2 in relation to cells' polarization. Here we propose a methodology that combines immunofluorescent staining with quantitative imaging to prepare maps of protein distribution frequency that can serve to compare protein distribution on nuclear membrane.

Indexed as

Nuclear EnvelopeNuclear ProteinsAnimalsFluorescent Antibody TechniqueHumansLamin Type AMembrane ProteinsemerinLamin Type AMembrane ProteinsNuclear ProteinsConfocal microscopyFrequency distribution mapNuclear envelopeNuclear membraneQuantitative imaging

Identifiers

PMID40833571

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.